Impedimetric method for measuring ultra-low E. coli concentrations in human urine

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题名:	Impedimetric method for measuring ultra-low E. coli concentrations in human urine
作者:	蔡章仁;Settu, Kalpana;Chen, Ching-Jung;Liu, Jen-Tsai;Chen, Chien-Lung;Tsai, Jang-Zern
贡献者:	資訊電機學院電機工程學系
关键词:	Bacteria;bacterial adhesion;Biofilm;Biofilms;Biosensing Techniques - methods;Biosensors;Capacitance;Electric circuits;Electric Impedance;Electrochemical impedance spectroscopy;Electrodes;electronic circuits;Escherichia coli;Escherichia coli - isolation & purification;gold;Gold - chemistry;Human;Humans;Impedance;Interdigitated microelectrode;microbial growth;Microelectrodes;Sensors;urinary tract diseases;Urinary tract infection (UTI);Urine;Urine - microbiology
日期:	2015-04-05
上传时间:	2026-04-23 14:14:21 (UTC+8)
出版者:	Elsevier Ltd.;England: Elsevier B.V
摘要:	摘要： In this study, we developed an interdigitated gold microelectrode-based impedance sensor to detect Escherichia coli (E. coli) in human urine samples for urinary tract infection (UTI) diagnosis. E. coli growth in human urine samples was successfully monitored during a 12-h culture, and the results showed that the maximum relative changes could be measured at 10Hz. An equivalent electrical circuit model was used for evaluating the variations in impedance characteristics of bacterial growth. The equivalent circuit analysis indicated that the change in impedance values at low frequencies was caused by double layer capacitance due to bacterial attachment and formation of biofilm on electrode surface in urine. A linear relationship between the impedance change and initial E. coli concentration was obtained with the coefficient of determination R2>0.90 at various growth times of 1, 3, 5, 7, 9 and 12h in urine. Thus our sensor is capable of detecting a wide range of E. coli concentration, 7×100 to 7×108 cells/ml, in urine samples with high sensitivity. •This study reports the possibility of detecting E. coli in urine samples using interdigitated microelectrode sensor.•Ultra-low E. coli concentration was measured in urine by growth time-graded impedance monitoring approach.•Sensor was capable of detecting larger range of E. coli concentrations at various growth times.•Merits of the method include easy fabrication, low-cost and user-friendly. 其他題名： Biosens Bioelectron 出版者： England: Elsevier B.V 出版日期： 2015-04-15 出處： Biosensors & bioelectronics, 2015-04, Vol.66, p.244-250 版權： 2014 Elsevier B.V. 版權： Copyright © 2014 Elsevier B.V. All rights reserved. 識別號： ISSN: 0956-5663 識別號： ISSN: 1873-4235 識別號： EISSN: 1873-4235 識別號： DOI: 10.1016/j.bios.2014.11.027 識別號： PMID: 25437359
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