Screen-printed carbon electrode-based electrochemical immunosensor for rapid detection of microalbuminuria

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題名:	Screen-printed carbon electrode-based electrochemical immunosensor for rapid detection of microalbuminuria
作者:	蔡章仁;Tsai, Jang-Zern;Chen, Ching-Jung;Settu, Kalpana;Lin, Yu-Feng;Chen, Chien-Lung;Liu, Jen-Tsai
貢獻者:	資訊電機學院電機工程學系
關鍵詞:	Albumins;Albumins - analysis;albuminuria;Albuminuria - diagnosis;Albuminuria - urine;antibodies;ascorbic acid;Biosensing Techniques - instrumentation;Biosensors;Carbon;Carbon - chemistry;Chronoamperometry;Conductometry - instrumentation;creatinine;detection limit;diabetes;electrochemistry;Electrodes;energy-dispersive X-ray analysis;Equipment Design;Equipment Failure Analysis;glucose;Humans;hypertension;Immunoassay - instrumentation;Immunosensor;Immunosensors;kidney diseases;Kidneys;Microalbuminuria;people;Photography - methods;rapid methods;Risk;scanning electron microscopy;Screen-printed carbon electrode;screening;Uric acid;Urinalysis - instrumentation;Urine
日期:	2016-03-15
上傳時間:	2026-04-23 14:37:46 (UTC+8)
出版者:	Elsevier Ltd.;England: Elsevier B.V
摘要:	摘要： A urinary microalbumin test is used to detect early signs of kidney damage in people who have a risk of chronic kidney disease, such as those with diabetes or hypertension. In this study, we developed a screen-printed carbon electrode-based immunosensor for the detection of microalbumin in urine. Anti-human albumin antibodies were immobilized on the screen-printed sensor surface by the covalent immobilization method. Cyclic voltammetry (CV) and scanning electron microscopy with an energy dispersive spectroscopical (SEM-EDS) analysis demonstrated that the modification process was well performed. Chronoamperometric (CA) electrochemical measurement technique was employed for the quantitative detection of albumin. The electrochemical measurements performed with some possible interfering compounds normally present in urine (ascorbic acid, uric acid, glucose and creatinine samples) demonstrated a high specificity and selectivity of this immunosensor in albumin detection. Under optimum conditions, the immunosensor can detect urinary albumin in a wide linear range from 10µg/ml to 300µg/ml with a detection limit of 9.7µg/ml. The excellent performance of this immunosensor was confirmed by analyzing microalbumin in urine samples; the results were in good agreement with those obtained by the standard immunoturbidimetric method. The biosensor proposed herein is easy to prepare and can be used for low-cost, rapid, and sensitive screening of microalbuminuria. This approach provides a promising platform for developing clinical point-of-care diagnostic applications. •This immunosensor can detect urinary albumin in a wide range from 10 to 300µg/ml.•This immunosensor is compatible with standard clinical methods.•This immunosensor is easy to fabricate, low-cost, and user-friendly.•This method could be used for developing point-of-care diagnostic applications. 其他題名： Biosens Bioelectron 出版者： England: Elsevier B.V 出版日期： 2016-03-15 出處： Biosensors & bioelectronics, 2016-03, Vol.77, p.1175-1182 版權： 2015 Elsevier B.V. 版權： Copyright © 2015 Elsevier B.V. All rights reserved. 識別號： ISSN: 0956-5663 識別號： ISSN: 1873-4235 識別號： EISSN: 1873-4235 識別號： DOI: 10.1016/j.bios.2015.11.002 識別號： PMID: 26579935
顯示於類別:	[電機工程學系] 期刊論文

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