Silicon Quantum Dot Nanoparticles with Antifouling Coatings for Immunostaining on Live Cancer Cells

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題名:	Silicon Quantum Dot Nanoparticles with Antifouling Coatings for Immunostaining on Live Cancer Cells
作者:	杜長慶;Tu, Chang-Ching;Chen, Kuang-Po;Yang, Tsu-An;Chou, Min-Yuan;Lin, Lih Y;Li, Yaw-Kuen
貢獻者:	資訊電機學院電機工程學系
關鍵詞:	antibodies;antifouling agents;biocompatibility;biodegradability;bovine serum albumin;cell viability;cytotoxicity;electrochemistry;energy;flow cytometry;fluorescein;fluorescence;fluorescent antibody technique;hydrophilicity;image analysis;isothiocyanates;milling;nanoparticles;neoplasm cells;neoplasms;nitric acid;photoluminescence;polyethylene glycol;quantum dots;silicon;solvents;water solubility
日期:	2016-06-08
上傳時間:	2026-04-23 14:39:51 (UTC+8)
出版者:	American Chemical Society;United States: American Chemical Society
摘要:	摘要： Fluorescent silicon quantum dots (SiQDs) have shown a great potential as antiphotobleaching, nontoxic and biodegradable labels for various in vitro and in vivo applications. However, fabricating SiQDs with high water-solubility and high photoluminescence quantum yield (PLQY) remains a challenge. Furthermore, for targeted imaging, their surface chemistry has to be capable of conjugating to antibodies, as well as sufficiently antifouling. Herein, antibody-conjugated SiQD nanoparticles (SiQD-NPs) with antifouling coatings composed of bovine serum albumin (BSA) and polyethylene glycol (PEG) are demonstrated for immunostaining on live cancer cells. The monodisperse SiQD-NPs of diameter about 130 nm are synthesized by a novel top-down method, including electrochemical etching, photochemical hydrosilylation, high energy ball milling, and “selective-etching” in HNO3 and HF. Subsequently, the BSA and PEG are covalently grafted on to the SiQD-NP surface through presynthesized chemical linkers, resulting in a stable, hydrophilic, and antifouling organic capping layer with isothiocyanates as the terminal functional groups for facile conjugation to the antibodies. The in vitro cell viability assay reveals that the BSA-coated SiQD-NPs had exceptional biocompatibility, with minimal cytotoxicity at concentration up to 1600 μg mL–1. Under 365 nm excitation, the SiQD-NP colloid emits bright reddish photoluminescence with PLQY = 45–55% in organic solvent and 5–10% in aqueous buffer. Finally, through confocal fluorescent imaging and flow cytometry analysis, the anti-HER2 conjugated SiQD-NPs show obvious specific binding to the HER2-overexpressing SKOV3 cells and negligible nonspecific binding to the HER2-nonexpressing CHO cells. Under similar experimental conditions, the immunofluorescence results obtained with the SiQD-NPs are comparable to those using conventional fluorescein isothiocyanate (FITC). 其他題名： ACS Appl. Mater. Interfaces 出版者： United States: American Chemical Society 出版日期： 2016-06-08 出處： ACS applied materials & interfaces, 2016-06, Vol.8 (22), p.13714-13723 資源來源： American Chemical Society Journals 版權： Copyright © 2016 American Chemical Society 識別號： ISSN: 1944-8244 識別號： ISSN: 1944-8252 識別號： EISSN: 1944-8252 識別號： DOI: 10.1021/acsami.6b02318 識別號： PMID: 27198164
顯示於類別:	[電機工程學系] 期刊論文

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