The influence of NBD fluorescent probe on model membranes containing POPC and DPPC

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/108249

Title:	The influence of NBD fluorescent probe on model membranes containing POPC and DPPC
Authors:	薛雅薇;Weng, Chi-Jung;Wu, Ju-Ping;Kuo, Ming-Yen;Hsueh, Ya-Wei
Contributors:	理學院物理學系
Keywords:	1,2-Dipalmitoylphosphatidylcholine - analogs & derivatives;1,2-Dipalmitoylphosphatidylcholine - chemistry;2H NMR;Azoles - chemistry;C6-NBD-PC;DPPC;fluorescence microscopy;Fluorescent Dyes - chemistry;Glycerophospholipids - chemistry;Glycerylphosphorylcholine - analogs & derivatives;Glycerylphosphorylcholine - chemistry;Membranes - chemistry;Membranes - ultrastructure;NBD-DOPE;Nitrobenzenes - chemistry;POPC;Proton Magnetic Resonance Spectroscopy;Temperature;Unilamellar Liposomes - chemistry
Date:	2016-02-17
Issue Date:	2026-04-23 14:40:25 (UTC+8)
Publisher:	Informa Healthcare;England: Taylor & Francis
Abstract:	摘要： To investigate the effect of fluorescent probe on the properties of membranes, we studied model membranes composed of 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl 2-oleoyl-sn-glycero-3-phosphocholine (POPC) in the presence and absence of fluorescent probe. The morphology of giant unilamellar vesicles (GUVs) has been observed as a function of temperature and composition by fluorescence microscopy using NBD-DOPE or C 6 -NBD-PC as the probe. The phase behavior of model membranes containing no fluorescent probe was investigated by 2 H-NMR spectroscopy. We found that the bright phase observed on GUVs was the fluid phase enriched in POPC and the dark phase was the gel phase enriched in DPPC. NBD-DOPE and C 6 -NBD-PC preferentially participated in the fluid-phase domains when GUVs were in the gel + fluid phase coexistence. Inclusion of both fluorescent probes (1 mol%) lowered the transition temperature of POPC/DPPC membranes. In addition, C 6 -NBD-PC exhibited a stronger effect than NBD-DOPE, which was considered to be associated with the structures of fluorescent molecules. 其他題名： Mol Membr Biol 出版者： England: Taylor & Francis 出版日期： 2016-02-17 出處： Molecular membrane biology, 2016-02, Vol.33 (1-2), p.23-28 資源來源： EBSCOhost Academic Search Premier 版權： 2016 Informa UK Limited, trading as Taylor & Francis Group 2016 識別號： ISSN: 0968-7688 識別號： ISSN: 1464-5203 識別號： EISSN: 1464-5203 識別號： DOI: 10.1080/09687688.2016.1185175 識別號： PMID: 27452979
Appears in Collections:	[Department of Physics] journal & Dissertation

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