Three-dimensional spherical spatial boundary conditions differentially regulate osteogenic differentiation of mesenchymal stromal cells

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題名:	Three-dimensional spherical spatial boundary conditions differentially regulate osteogenic differentiation of mesenchymal stromal cells
作者:	林耿慧;Lo, Yin-Ping;Liu, Yi-Shiuan;Rimando, Marilyn G.;Ho, Jennifer Hui-Chun;Lin, Keng-hui;Lee, Oscar K.
貢獻者:	理學院物理學系
關鍵詞:	13/100;13/109;13/89;14/19;14/34;14/63;38;38/22;38/77;38/90;42/41;631/1647/767/2200;631/532/1360;631/532/2118/2074;631/532/2139;Actin;Actins - metabolism;Boundary conditions;Cell Adhesion;Cell culture;Cell Culture Techniques;Cell Differentiation;Cell Survival;Cytoskeleton;Focal Adhesions;Homeostasis;Humanities and Social Sciences;Humans;Integrin alpha2 - metabolism;Integrin alpha5 - metabolism;Integrins;Mesenchymal stem cells;Mesenchymal Stromal Cells - cytology;Mesenchyme;multidisciplinary;Organogenesis;Osteogenesis;Regenerative medicine;Science;Skin & tissue grafts;Spatial distribution;Spheroids, Cellular;Stem Cell Niche;Stromal cells;Tissue engineering;Tissue Scaffolds
日期:	2016-02-17
上傳時間:	2026-04-23 14:43:25 (UTC+8)
出版者:	Nature Publishing Group;London: Nature Publishing Group UK
摘要:	摘要： The spatial boundary condition (SBC) arising from the surrounding microenvironment imposes specific geometry and spatial constraints that affect organogenesis and tissue homeostasis. Mesenchymal stromal cells (MSCs) sensitively respond to alterations of mechanical cues generated from the SBC. However, mechanical cues provided by a three-dimensional (3D) environment are deprived in a reductionist 2D culture system. This study investigates how SBC affects osteogenic differentiation of MSCs using 3D scaffolds with monodispersed pores and homogenous spherical geometries. MSCs cultured under SBCs with diameters of 100 and 150 μm possessed the greatest capability of osteogenic differentiation. This phenomenon was strongly correlated with MSC morphology, organization of actin cytoskeleton and distribution of focal adhesion involving α2 and α5 integrins. Further silencing either α2 or α5 integrin significantly reduced the above mentioned mechanosensitivity, indicating that the α2 and α5 integrins as mechano-sensitive molecules mediate MSCs’ ability to provide enhanced osteogenic differentiation in response to different spherical SBCs. Taken together, the findings provide new insights regarding how MSCs respond to mechanical cues from the surrounding microenvironment in a spherical SBC and such biophysical stimuli should be taken into consideration in tissue engineering and regenerative medicine in conjunction with biochemical cues. 其他題名： Sci Rep 出版者： London: Nature Publishing Group UK 出版日期： 2016-02-17 出處： Scientific reports, 2016-02, Vol.6 (1), p.21253, Article 21253 資源來源： Publicly Available Content Database 版權： The Author(s) 2016 版權： Copyright Nature Publishing Group Feb 2016 版權： Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited 識別號： ISSN: 2045-2322 識別號： EISSN: 2045-2322 識別號： DOI: 10.1038/srep21253 識別號： PMID: 26884253
顯示於類別:	[物理學系] 期刊論文

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