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| 題名: | Anaerobic and aerobic cleavage of the steroid core ring structure by Steroidobacter denitrifi cans |
| 作者: | 王柏翔;Wang, Po-Hsiang;Leu, Yann-Lii;Ismail, Wael;Tang, Sen-Lin;Tsai, Ching-Yen;Chen, Hsing-Ju;Kao, Ann-Tee;Chiang, Yin-Ru |
| 貢獻者: | 工學院環境工程研究所 |
| 關鍵詞: | Aerobiosis;anaerobic metabolism;Anaerobiosis;Biodegradation, Environmental;denitrifying bacteria;Gammaproteobacteria - chemistry;Gammaproteobacteria - metabolism;Humans;metabolomics;Oxidation-Reduction;Oxygen - metabolism;Patient-Oriented and Epidemiological Research;steroid biodegradation;Steroids - chemistry;Steroids - metabolism;testosterone;Testosterone - chemistry;Testosterone - metabolism |
| 日期: | 2013-05-01 |
| 上傳時間: | 2026-04-23 14:59:00 (UTC+8) |
| 出版者: | American Society for Biochemistry and Molecular Biology Inc.;United States: Elsevier Inc |
| 摘要: | 摘要： The aerobic degradation of steroids by bacteria has been studied in some detail. In contrast, only little is known about the anaerobic steroid catabolism. Steroidobacter denitrificans can utilize testosterone under both oxic and anoxic conditions. By conducting metabolomic investigations, we demonstrated that S. denitrificans adopts the 9,10-seco-pathway to degrade testosterone under oxic conditions. This pathway depends on the use of oxygenases for oxygenolytic ring fission. Conversely, the detected degradation intermediates under anoxic conditions suggest a novel, oxygenase-independent testosterone catabolic pathway, the 2,3-seco-pathway, which differs significantly from the aerobic route. In this anaerobic pathway, testosterone is first transformed to 1-dehydrotestosterone, which is then reduced to produce 1-testosterone followed by water addition to the C-1/C-2 double bond of 1-testosterone. Subsequently, the C-1 hydroxyl group is oxidized to produce 17-hydroxy-androstan-1,3-dione. The A-ring of this compound is cleaved by hydrolysis as evidenced by H218O-incorporation experiments. Regardless of the growth conditions, testosterone is initially transformed to 1-dehydrotestosterone. This intermediate is a divergence point at which the downstream degradation pathway is governed by oxygen availability. Our results shed light into the previously unknown cleavage of the sterane ring structure without oxygen. We show that, under anoxic conditions, the microbial cleavage of steroidal core ring system begins at the A-ring.
其他題名： J Lipid Res
出版者： United States: Elsevier Inc
出版日期： 2013-05
出處： Journal of lipid research, 2013-05, Vol.54 (5), p.1493-1504
資源來源： Alma/SFX Local Collection
版權： 2013 © 2013 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.
版權： Copyright © 2013 by the American Society for Biochemistry and Molecular Biology, Inc. 2013
識別號： ISSN: 0022-2275
識別號： ISSN: 1539-7262
識別號： EISSN: 1539-7262
識別號： DOI: 10.1194/jlr.M034223
識別號： PMID: 23458847 |
| 顯示於類別: | [環境工程研究所 ] 期刊論文
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