Development of a pneumatically driven active cover lid for multi-well microplates for use in perfusion three-dimensional cell culture

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题名:	Development of a pneumatically driven active cover lid for multi-well microplates for use in perfusion three-dimensional cell culture
作者:	周鼎贏;Huang, Song-Bin;Chou, Dean;Chang, Yu-Han;Li, Ke-Cing;Chiu, Tzu-Keng;Ventikos, Yiannis;Wu, Min-Hsien
贡献者:	工學院機械工程學系
关键词:	13;13/62;631/61/32;639/166/985;Animals;Bioassays;Cell culture;Cell Culture Techniques - instrumentation;Cell Culture Techniques - methods;Culture techniques;Flow rates;Humanities and Social Sciences;Humans;Mesenchymal Stromal Cells - cytology;Mesenchymal Stromal Cells - metabolism;Mesenchyme;Microfluidics;multidisciplinary;Perfusion;Physiology;Science;Stem cells
日期:	2015-12-16
上传时间:	2026-04-23 14:59:02 (UTC+8)
出版者:	Nature Publishing Group;London: Nature Publishing Group UK
摘要:	摘要： Before microfluidic-based cell culture models can be practically utilized for bioassays, there is a need for a transitional cell culture technique that can improve conventional cell culture models. To address this, a hybrid cell culture system integrating an active cover lid and a multi-well microplate was proposed to achieve perfusion 3-D cell culture. In this system, a microfluidic-based pneumatically-driven liquid transport mechanism was integrated into the active cover lid to realize 6-unit culture medium perfusion. Experimental results revealed that the flow of culture medium could be pneumatically driven in a flow-rate uniform manner. We used the system to successfully perform a perfusion 3-D cell culture of mesenchymal stem cells (MSCs) for up to 16 days. Moreover, we investigated the effects of various cell culture models on the physiology of MSCs. The physiological nature of MSCs can vary with respect to the cell culture model used. Using the perfusion 3-D cell culture format might affect the proliferation and osteogenic differentiation of MSCs. Overall, we have developed a cell culture system that can achieve multi-well microplate-based perfusion 3-D cell culture in an efficient, cost-effective and user-friendly manner. These features could facilitate the widespread application of perfusion cell culture models for cell-based assays. 其他題名： Sci Rep 出版者： London: Nature Publishing Group UK 出版日期： 2015-12-16 出處： Scientific reports, 2015-12, Vol.5 (1), p.18352, Article 18352 資源來源： Publicly Available Content Database (Proquest) 版權： The Author(s) 2015 版權： Copyright Nature Publishing Group Dec 2015 版權： Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited 識別號： ISSN: 2045-2322 識別號： EISSN: 2045-2322 識別號： DOI: 10.1038/srep18352 識別號： PMID: 26669749
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