Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment

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Title:	Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment
Authors:	王柏翔;Yang, Fu-Chun;Chen, Yi-Lung;Tang, Sen-Lin;Yu, Chang-Ping;Wang, Po-Hsiang;Ismail, Wael;Wang, Chia-Hsiang;Ding, Jiun-Yan;Yang, Cheng-Yu;Yang, Chia-Ying;Chiang, Yin-Ru
Contributors:	工學院環境工程研究所
Keywords:	38/43;38/91;45/23;45/77;631/158/855;82/16;82/83;Anaerobiosis;Androgens;Androgens - metabolism;Bacteria;Biodegradation;Biodegradation, Environmental;Biomarkers;Biomedical and Life Sciences;Complex compounds;Contaminants;Dehydrogenase;Dehydrogenases;Denitrifying bacteria;Ecology;Evolutionary Biology;Gammaproteobacteria - genetics;Gammaproteobacteria - metabolism;Gene clusters;Gene Expression Profiling;Genome, Bacterial - genetics;Genomics;Hormones;Life Sciences;Metabolites;Metagenomics;Microbial degradation;Microbial Ecology;Microbial Genetics and Genomics;Microbiology;Microorganisms;Original;original-article;Phylogeny;Sequence Analysis, DNA;Sewage - microbiology;Sewage treatment;Sewage treatment plants;Steroid hormones;Steroids;Substrate preferences;Surface water;Taiwan;Testosterone;Testosterone - analogs & derivatives;Testosterone - metabolism;Thauera;Transcriptomics;Wastewater treatment plants;Water pollution
Date:	2016-08-01
Issue Date:	2026-04-23 15:04:22 (UTC+8)
Publisher:	Nature Publishing Group;London: Nature Publishing Group UK
Abstract:	摘要： Steroid hormones, such as androgens, are common surface-water contaminants. However, literature on the ecophysiological relevance of steroid-degrading organisms in the environment, particularly in anoxic ecosystems, is extremely limited. We previously reported that Steroidobacter denitrificans anaerobically degrades androgens through the 2,3- seco pathway. In this study, the genome of Sdo. denitrificans was completely sequenced. Transcriptomic data revealed gene clusters that were distinctly expressed during anaerobic growth on testosterone. We isolated and characterized the bifunctional 1-testosterone hydratase/dehydrogenase, which is essential for anaerobic degradation of steroid A-ring. Because of apparent substrate preference of this molybdoenzyme, corresponding genes, along with the signature metabolites of the 2,3- seco pathway, were used as biomarkers to investigate androgen biodegradation in the largest sewage treatment plant in Taipei, Taiwan. Androgen metabolite analysis indicated that denitrifying bacteria in anoxic sewage use the 2,3- seco pathway to degrade androgens. Metagenomic analysis and PCR-based functional assays showed androgen degradation in anoxic sewage by Thauera spp. through the action of 1-testosterone hydratase/dehydrogenase. Our integrative ‘omics’ approach can be used for culture-independent investigations of the microbial degradation of structurally complex compounds where isotope-labeled substrates are not easily available. 其他題名： ISME J 出版者： London: Nature Publishing Group UK 出版日期： 2016-08-01 出處： The ISME Journal, 2016-08, Vol.10 (8), p.1967-1983 資源來源： Agricultural & Environmental Science Collection 版權： International Society for Microbial Ecology 2016 版權： Copyright Nature Publishing Group Aug 2016 版權： 2016 International Society for Microbial Ecology 2016 版權： Copyright © 2016 International Society for Microbial Ecology 2016 International Society for Microbial Ecology 識別號： ISSN: 1751-7362 識別號： ISSN: 1751-7370 識別號： EISSN: 1751-7370 識別號： DOI: 10.1038/ismej.2015.255 識別號： PMID: 26872041
Appears in Collections:	[Graduate Institute of Environmental Engineering ] journal & Dissertation

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