Electrical stimulation to promote osteogenesis using conductive polypyrrole films

NCUIR > Engineering College > Departmant of Mechanical Engineering > journal & Dissertation > Item 987654321/108781

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/108781

Title:	Electrical stimulation to promote osteogenesis using conductive polypyrrole films
Authors:	曹嘉文;Hu, Wei-Wen;Hsu, Yi-Ting;Cheng, Yu-Che;Li, Chuan;Ruaan, Ruoh-Chyu;Chien, Chih-Cheng;Chung, Chih-Ang;Tsao, Chia-Wen
Contributors:	工學院機械工程學系
Keywords:	Animals;Biocompatible Materials - chemistry;Bone Marrow Cells - cytology;Bone marrow stromal cells;Cell Differentiation;Cells, Cultured;Direct current field;Electric Stimulation;Electrical stimulation;Mesenchymal Stromal Cells - cytology;Mineralization;Osteogenesis;Osteogenesis - physiology;Polymers - chemistry;Polypyrrole;Pyrroles - chemistry;Rats
Date:	2014-04-01
Issue Date:	2026-04-23 15:05:58 (UTC+8)
Publisher:	Elsevier BV;Netherlands: Elsevier B.V
Abstract:	摘要： In this study, we developed an electrical cell culture and monitoring device. Polypyrrole (PPy) films with different resistances were fabricated as conductive surfaces to investigate the effect of substrate-mediated electrical stimulation. The physical and chemical properties of the devices, as well as their biocompatibilities, were thoroughly evaluated. These PPy films had a dark but transparent appearance, on which the surface cells could be easily observed. After treating with the osteogenic medium, rat bone marrow stromal cells cultured on the PPy films differentiated into osteoblasts. The cells grown on the PPy films had up-regulated osteogenic markers, and an alkaline phosphatase activity assay showed that the PPy films accelerated cell differentiation. Alizarin red staining and calcium analysis suggested that the PPy films promoted osteogenesis. Finally, PPy films were subjected to a constant electric field to elucidate the effect of electrical stimulation on osteogenesis. Compared with the untreated group, electrical stimulation improved calcium deposition in the extracellular matrix. Furthermore, PPy films with lower resistances allowed larger currents to stimulate the surface cells, which resulted in higher levels of mineralization. Overall, these results indicated that this system exhibited superior electroactivity with controllable electrical resistance and that it can be coated directly to produce medical devices with a transparent appearance, which should be beneficial for research on electrical stimulation for tissue regeneration. [Display omitted] •Polypyrrole (PPy) with adjustable resistance demonstrates good biocompatibility.•Conductive PPy films can be deposited to surfaces with transparent appearances.•PPy films accelerate the differentiation of rat bone marrow stromal cells (rBMSCs).•PPy incorporated electrical devices highly improve the mineralization of rBMSCs. 其他題名： Mater Sci Eng C Mater Biol Appl 出版者： Netherlands: Elsevier B.V 出版日期： 2014-04-01 出處： Materials Science & Engineering C, 2014-04, Vol.37, p.28-36 資源來源： Elsevier ScienceDirect Journals Complete 版權： 2013 Elsevier B.V. 版權： Copyright © 2013 Elsevier B.V. All rights reserved. 識別號： ISSN: 0928-4931 識別號： ISSN: 1873-0191 識別號： EISSN: 1873-0191 識別號： DOI: 10.1016/j.msec.2013.12.019 識別號： PMID: 24582219
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