Peptide synthesis on glass substrate using acoustic droplet ejector

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题名:	Peptide synthesis on glass substrate using acoustic droplet ejector
作者:	陳世叡;Choe, Youngki;Chen, Shih-Jui;Kim, Eun Sok
贡献者:	工學院機械工程學系
关键词:	Acoustics;Amino acids;Biomedical measurement;Couplings;Droplet ejector;Equipment Design;Glass;Liquids;Nanotechnology - instrumentation;Nanotechnology - methods;nozzleless droplet ejector;peptide synthesis;Peptides;Peptides - chemical synthesis;Peptides - chemistry;Protein Array Analysis - instrumentation;protein synthesis;SPOT synthesis;Surface Properties
日期:	2014-03-01
上传时间:	2026-04-23 15:22:19 (UTC+8)
出版者:	IEEE Computer Society;United States: IEEE
摘要:	摘要： This paper describes the synthesis of a 9-mers-long peptide ladder structure of glycine on a modified glass surface using a nanoliter droplet ejector. To synthesize peptide on a glass substrate, SPOT peptide synthesis protocol was followed with a nozzleless acoustic droplet ejector being used to eject about 300 droplets of preactivated amino acid solution to dispense 60 nL of the solution per mer. The coupling efficiency of each mer was measured with FITC fluorescent tag to be 96%, resulting in net 70% efficiency for the whole 9-mer-long peptide of glycine. Usage of a nanoliter droplet ejector for SPOT peptide synthesis increases the density of protein array on a chip. 其他題名： TBME 其他題名： IEEE Trans Biomed Eng 出版者： United States: IEEE 出版日期： 2014-03 出處： IEEE transactions on biomedical engineering, 2014-03, Vol.61 (3), p.705-710 資源來源： IEEE Electronic Library (IEL) 版權： Copyright The Institute of Electrical and Electronics Engineers, Inc. (IEEE) Mar 2014 識別號： ISSN: 0018-9294 識別號： ISSN: 1558-2531 識別號： EISSN: 1558-2531 識別號： DOI: 10.1109/TBME.2013.2287218 識別號： PMID: 24235271 識別號： CODEN: IEBEAX
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