Solid freeform-fabricated scaffolds designed to carry multicellular mesenchymal stem cell spheroids for cartilage regeneration

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题名:	Solid freeform-fabricated scaffolds designed to carry multicellular mesenchymal stem cell spheroids for cartilage regeneration
作者:	曾清秀;Huang, G-S;Tseng, C-S;Linju Yen, B;Dai, L-G;Hsieh, P-S;Hsu, S-h
贡献者:	工學院機械工程學系
关键词:	Adult;Animals;Biodegradable Plastics - chemistry;Biodegradable Plastics - pharmacology;Cartilage - physiology;cartilage regeneration;Cell Adhesion - drug effects;Cell Movement - drug effects;Cellular Microenvironment - drug effects;chitosan;Chitosan - chemistry;Chitosan - pharmacology;Extracellular Matrix - drug effects;Female;Humans;mesenchymal stem cell spheroids;Mesenchymal Stem Cell Transplantation;Mesenchymal Stem Cells - drug effects;Mesenchymal Stem Cells - physiology;Mice;Mice, Inbred NOD;Mice, SCID;Polyglactin 910 - chemistry;Polyglactin 910 - pharmacology;Rabbits;Regeneration;scaffolds;Solid freeform fabrication;Spheroids, Cellular - drug effects;Spheroids, Cellular - physiology;Tissue Scaffolds - chemistry
日期:	2013-10-13
上传时间:	2026-04-23 15:27:51 (UTC+8)
出版者:	AO Research Institute;Switzerland: Forum Multimedia Publishing LLC
摘要:	摘要： Three-dimensional (3D) cellular spheroids have recently emerged as a new trend to replace suspended single cells in modern cell-based therapies because of their greater regeneration capacities in vitro. They may lose the 3D structure during a change of microenvironment, which poses challenges to their translation in vivo. Besides, the conventional microporous scaffolds may have difficulty in accommodating these relatively large spheroids. Here we revealed a novel design of microenvironment for delivering and sustaining the 3D spheroids. Biodegradable scaffolds with macroporosity to accommodate mesenchymal stem cell (MSC) spheroids were made by solid freeform fabrication (SFF) from the solution of poly(D,L-lactide-co-glycolide). Their internal surface was modified with chitosan following air plasma treatment in order to preserve the morphology of the spheroids. It was demonstrated that human MSC spheroids loaded in SFF scaffolds produced a significantly larger amount of cartilage-associated extracellular matrix in vitro and in NOD/SCID mice compared to single cells in the same scaffolds. Implantation of MSC spheroid-loaded scaffolds into the chondral defects of rabbit knees showed superior cartilage regeneration. This study establishes new perspectives in designing the spheroid-sustaining microenvironment within a tissue engineering scaffold for in vivo applications. 其他題名： Eur Cell Mater 出版者： Switzerland: Forum Multimedia Publishing LLC 出版日期： 2013-10-13 出處： European Cells and Materials, 2013-10, Vol.26, p.179-194 資源來源： DOAJ Directory of Open Access Journals 識別號： ISSN: 1473-2262 識別號： EISSN: 1473-2262 識別號： DOI: 10.22203/eCM.v026a13 識別號： PMID: 24122653
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