The control of cell orientation using biodegradable alginate fibers fabricated by near-field electrospinning

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題名:	The control of cell orientation using biodegradable alginate fibers fabricated by near-field electrospinning
作者:	傅尹坤;Fuh, Yiin-Kuen;Wu, Yun-Chung;He, Zhe-Yu;Huang, Zih-Ming;Hu, Wei-Wen
貢獻者:	工學院機械工程學系
關鍵詞:	Alginate;Alginates;Alginates - chemistry;Alignment;Biocompatibility;Biocompatible Materials - chemistry;Biocompatible Materials - pharmacology;Biodegradation;Cell Adhesion - drug effects;Cell alignment;Cell Survival - drug effects;Control systems;Crosslinking;Degradation;Electrospinning;Fibers;Glucuronic Acid - chemistry;HEK293 Cells;Hexuronic Acids - chemistry;Humans;Near-field electrospinning;Orientation;Polyethylene Glycols - chemistry
日期:	2016-05-01
上傳時間:	2026-04-23 15:29:31 (UTC+8)
出版者:	Elsevier BV;Netherlands: Elsevier B.V
摘要:	摘要： For spatially controlling cell alignment, near field electrospinning (NFES) was developed to direct-write alginate fiber patterns. Compared to randomly electrospun fibers, NFES fibers guided the extension of HEK 293T cells and the levels of cell alignment increased with decreasing fiber distances. However, these guiding fibers were unfavorable for cell adhesion and limited cell growth. To preserve cell alignment ability and improve biocompatibility, the stability of patterned alginate fibers was adjusted by regulating the level of ion crosslinking. These partially crosslinked NFES fibers demonstrated parallel line-patterns in the initial stage while gradually degraded with time. The reduction of fiber density increased the available area for cell growth and enhanced cell viability. On the other hand, aligned cells were still found on these degraded patterns, suggesting that cell morphologies were mainly guided during cell seeding. This dynamically controlled fiber pattern system fulfilled the need of controlling cell orientation and biocompatibility, thus was potential to modify scaffold surfaces for tissue engineering application. [Display omitted] •Alginate fiber patterns were directly written by near field electrospinning (NFES).•Shorter distances between parallel alginate lines resulted in higher cell alignments.•The stabilities of alginate patterns were regulated by the levels of crosslinking.•Degraded NFES patterns not only aligned surface cells but also improved cell growth. 其他題名： Mater Sci Eng C Mater Biol Appl 出版者： Netherlands: Elsevier B.V 出版日期： 2016-05-01 出處： Materials Science & Engineering C, 2016-05, Vol.62, p.879-887 資源來源： Elsevier ScienceDirect Journals Complete 版權： 2016 Elsevier B.V. 版權： Copyright © 2016 Elsevier B.V. All rights reserved. 識別號： ISSN: 0928-4931 識別號： EISSN: 1873-0191 識別號： DOI: 10.1016/j.msec.2016.02.028 識別號： PMID: 26952495
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