Toll-like receptor 6 and connective tissue growth factor are significantly upregulated in mitomycin-C-treated urothelial carcinoma cells under hydrostatic pressure stimulation

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Title:	Toll-like receptor 6 and connective tissue growth factor are significantly upregulated in mitomycin-C-treated urothelial carcinoma cells under hydrostatic pressure stimulation
Authors:	曹嘉文;Chen, Shao-Kuan;Chung, Chih-Ang;Cheng, Yu-Che;Huang, Chi-Jung;Chen, Wen-Yih;Ruaan, Ruoh-Chyu;Li, Chuan;Tsao, Chia-Wen;Hu, Wei-Wen;Chien, Chih-Cheng
Contributors:	工學院機械工程學系
Keywords:	Apoptosis;Cell Line, Tumor;Connective Tissue Growth Factor - genetics;Connective Tissue Growth Factor - physiology;Gene Expression;Humans;Hydrostatic Pressure;Mitomycin - pharmacology;Reverse Transcriptase Polymerase Chain Reaction;Toll-Like Receptor 6 - genetics;Toll-Like Receptor 6 - physiology;Up-Regulation;Urinary Bladder Neoplasms - metabolism;Urinary Bladder Neoplasms - pathology
Date:	2014-06-01
Issue Date:	2026-04-23 15:31:28 (UTC+8)
Publisher:	Mary Ann Liebert Inc.;Los Angeles, CA: SAGE Publications
Abstract:	摘要： Background: Urothelial carcinoma (UC) is the most common histologic subtype of bladder cancer. The administration of mitomycin C (MMC) into the bladder after transurethral resection of the bladder tumor (TURBT) is a common treatment strategy for preventing recurrence after surgery. We previously applied hydrostatic pressure combined with MMC in UC cells and found that hydrostatic pressure synergistically enhanced MMC-induced UC cell apoptosis through the Fas/FasL pathways. To understand the alteration of gene expressions in UC cells caused by hydrostatic pressure and MMC, oligonucleotide microarray was used to explore all the differentially expressed genes. Results: After bioinformatics analysis and gene annotation, Toll-like receptor 6 (TLR6) and connective tissue growth factor (CTGF) showed significant upregulation among altered genes, and their gene and protein expressions with each treatment of UC cells were validated by quantitative real-time PCR and immunoblotting. Conclusion: Under treatment with MMC and hydrostatic pressure, UC cells showed increasing apoptosis using extrinsic pathways through upregulation of TLR6 and CTGF. 其他題名： Genet Test Mol Biomarkers 出版者： Los Angeles, CA: SAGE Publications 出版日期： 2014-06-01 出處： Genetic testing and molecular biomarkers, 2014-06, Vol.18 (6), p.410-416 版權： Copyright 2014, Mary Ann Liebert, Inc. 識別號： ISSN: 1945-0265 識別號： ISSN: 1945-0257 識別號： EISSN: 1945-0257 識別號： DOI: 10.1089/gtmb.2013.0443 識別號： PMID: 24689870
Appears in Collections:	[Departmant of Mechanical Engineering ] journal & Dissertation

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