We report the optical study of microscopic particle motions and the dynamical behavior of free dislocations (FDs) in the strongly coupled quasi-two-dimensional dusty plasmas with SiO2 fine particle suspensions. The continuous excitation and relaxation of rotational cooperative particle motions with varying scales and lifetimes are observed, which distort the lattice and induce the FD processes such as generation, dissociation, propagation, recombination, and annihilation under the conservation of Burgers vector.