計畫目標: 藉與產業界合作, 研發出符合商業化生產巴西洋菇抗腫瘤物質之最佳培養基與操作條件.使得產程放大之技術得以在最短的時程內完成.並改進現有發酵產程, 使其產率再提高, 並使商品具有較高生理活性之特色.架構( 重要工作項目 ): 1.巴西洋菇液態培養之工業化培養基最適化條件探討.2.巴西洋菇液態培養之操作最適化條件探討.3.建立巴西洋菇抗腫瘤活性物質的分析技術.4.建立巴西洋菇多醣體促進機體免疫能力的分析方法.預期效益: 1.協助業界建立巴西洋菇生產抗腫瘤物質之培養基條件.2.協助業界尋求符合其發酵槽設計之最佳操作條件於巴西洋菇生產抗腫瘤物質.3.協助業界建立巴西洋菇抗腫瘤物質之有效性評估技術.4.協助研究人員認知業界之需要並達到人才培育之目的.使「保健食品」真正達到保健之目的. Background/Motivation: According to the recent statistical report about the causes of death of the people in Taiwan from the Department of Health, cancer has been on the top of the list.Health food market is increasing tremendously all over the world in the recent years.According a recent report of National Science Foundation in Taiwan, the health food market in Taiwan is estimated to be 23trillion NT per year.However, many health foods in the market are sold against the laws claimed to have certain healing effect toward certain illness without scientific evidence.As a result, many consumers become victims.”Health Food Control Act”was promulgated on February 3, 1999.And it should take effect on August 3, 1999.It is specified that the claims of health food should have the scientific evidence.Thus, it is necessary for academia to work together with industry to make an effort in the health food area for the welfare of consumers.Agaricus blazei was found to be one of the best mushrooms in the regards of antitumor activity.Recently, many anti-tumor materials and bioactive compounds have been isolated from Agaricus blazei.The major breakthrough for medicinal mushroom production is using submerged cultures to replace the conventional method, solid-state cultivation.There are many advantages of using submerged cultures over solid-state fermentation, which are faster growth rate, more polysaccharide production and no contamination by heavy metals.The operational conditions of bioreactors are essential for the production of functional polysaccharides from mycelium fermentation of Agaricus blazei.Nevertheless, little effort has been focused on the process parameters on the bioactivity of polysaccharides.Objectives: The major objectives of the project are to study the optimal commercial medium compositions for anti-tumor materials production by Agaricus blazei in the stirred-tank bioreactors and to search the optimal operational conditions for the large-scale bioreactors in Cyanamid Taiwan Corporation.It is expected to shorten the schedule of large-scale production of Agaricus blazei by integrating the remarkable results and experience of our laboratory.Project Structure: 1.Searching the optimal medium composition for industrial production of Agaricus blazei.2.Searching the optimal operational conditions for large-scale bioreactors in Cyanamid Taiwan Corporation.3.Building up the techniques for analysis of antitumor materials from the submerged culture of Agaricus blazei.4.Building up the bioassay techniques for antitumor polysaccharides from the submerged culture of Agaricus blazei.Expected Accomplishments: It is expected to accomplish some contributions toward the industry in this project.1.To help the industry to find out the optimal medium compositions for antitumor material production.2.To help the industry to find out the optimal operational conditions for their large-scale bioreactors.3.To help the industry to build up the bioassay techniques to evaluate their products from the developed media and operational conditions.I believe that both the industry and academia will be benefited from the project, and the consumers will be the final winners. 研究期間：9001 ~ 9012
