| 摘要: | (1) 建立巴西洋菇液態培養之產程最適化條件:操作條件最適化探討:方法:針對溫度、攪拌速度、pH值、通氣量等物理因素的改變對巴西洋菇菌株在生長、產物活性、產物結構、及產量等的影響進行探討. (2) 建立巴西洋菇液態培養之產程放大技術:產程放大策略探討:方法:以最適化培養基進行產程放大研究,由於有部分操作條件會隨著不同之反應器設計結構而改變,尤其是攪拌速率.因此將藉過去研發之成果與經驗來從事業者大型發酵槽之最適化條件探討.針對溫度、攪拌速度、pH值、通氣量等物理因素的改變對巴西洋菇菌株在生長、產物活性、產物結構、及產量等的影響進行探討,來建立產程放大策略. (3) 建立巴西洋菇之抗腫瘤多醣體之活性與其結構關聯性之分析技術:對於巴西洋菇之發酵產物進行下列細胞生理活性試驗: a. 抗腫瘤多醣體部份:方法:利用多醣體對RAW246.7免疫細胞之分泌TNF-a能力來鑑定其活性. b. 抗腫瘤多醣體之活性結構與組成部份:方法:先藉由GPC測多醣體之分子量大小或酵素法來處理,然後利用micro- filter分離與收集後,再分析其生物活性,如此應該可以尋求到活性多醣體之主要結構或分子量大小之訊息.本計畫之執行結果將達到以下效益: (1) 協助業界建立巴西洋菇生產抗腫瘤物質之培養基條件. (2) 協助業界尋求符合其發酵槽設計之最佳產程放大操作策略於巴西洋菇生產抗腫瘤物質. (3) 協助業界建立巴西洋菇抗腫瘤物質之有效性評估技術. (4) 協助研究人員認知業界之需要並達到人才培育之目的.使未來研發出之「保健食品」真正達到保健之目的。 Background/Motivation: According to the recent statistical report about the causes of death of the people in Taiwan from the Department of Health, cancer has been on the top of the list. Health food market is increasing tremendously all over the world in the recent years. According a recent report of National Science Foundation in Taiwan, the health food market in Taiwan is estimated to be 23 trillion NT per year. However, many health foods in the market are sold against the laws claimed to have certain healing effect toward certain illness without scientific evidence. As a result, many consumers become victims. Thus, the Legislation Yuan has pass the regulations for Health Food on January 3, 1999, which has been enforced by August in the same year. It is specified that the claims of health food should have the scientific evidence. Thus, it is necessary for academia to work together with industry to make an effort in the health food area for the welfare of consumers. Agaricus blazei was found to be one of the best mushrooms in the regards of antitumor activity. Recently, many anti-tumor materials and bioactive compounds have been isolated from Agaricus blazei. The major breakthrough for medicinal mushroom production is using submerged cultures to replace the conventional method, solid-state cultivation. There are many advantages of using submerged cultures over solid-state fermentation, which are faster growth rate, more polysaccharide production and no contamination by heavy metals. The operational conditions of bioreactors are essential for the production of functional polysaccharides from mycelium fermentation of Agaricus blazei. Nevertheless, little effort has been focused on the process parameters on the bioactivity of polysaccharides. Objectives: The major objectives of the project are to study the optimal commercial medium compositions for anti-tumor materials production by Agaricus blazei in the stirred-tank bioreactors and to search the optimal operational conditions for the large-scale bioreactors in Cyanamid Taiwan Corporation. It is expected to shorten the schedule of large-scale production of Agaricus blazei by integrating the remarkable results and experience of our laboratory. Project Structure: 1. Searching the optimal operational conditions for large-scale bioreactors in Cyanamid Taiwan Corporation. 2. Building up the enzymatic method and bioassay in analysis of antitumor materials from the submerged culture of Agaricus blazei. 3. Building up the membrane method and bioassay techniques for antitumor polysaccharides from the submerged culture of Agaricus blazei. Expected Accomplishments: It is expected to accomplish some contributions toward the industry in this project. 1. To help the industry to find out the optimal medium compositions for antitumor material production. 2. To help the industry to find out the optimal operational conditions for their large-scale bioreactors. 3. To help the industry to build up the bioassay techniques to evaluate their products from the developed media and operational conditions. I believe that both the industry and academia will be benefited from the project, and the consumers will be the final winners. 研究期間：9101 ~ 9112 |
