畜產品中Nitrofurans類之富來頓代謝物殘留檢驗試劑之開發 (I);+B3319Development of Screening Tests for Residues of Furazlidone in Animal Products (I)

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/42424

Title:	畜產品中Nitrofurans類之富來頓代謝物殘留檢驗試劑之開發 (I);+B3319Development of Screening Tests for Residues of Furazlidone in Animal Products (I)
Authors:	李文仁
Contributors:	化學學系
Keywords:	生物技術;畜牧獸醫類
Date:	2004-12-01
Issue Date:	2010-11-30 16:27:35 (UTC+8)
Publisher:	行政院農業委員會
Abstract:	計畫目標：開發畜產品中富來頓(Furazolidone)殘留檢驗試劑。本年度重要工作項目： 1. 富來頓(Furazolidone)藥物衍生物的製備：製備富來頓(Furazolidone)藥物的衍生物。 2. 富來頓(Furazolidone)藥物衍生物-蛋白質之結合體：完成富來頓(Furazolidone)藥物衍生物分別和不同蛋白質的結合。 3. 單株抗體動物免疫：以富來頓(Furazolidone)藥物衍生物-蛋白質結合體分別免疫小鼠，進行單株抗體之生產。 4. 免疫篩選系統之建立：建立ELISA免疫篩選系統，用來測定多株抗體免疫力價及單株抗體之篩選。 5. 細胞融合及單株抗體之篩選：挑選對對富來頓(Furazolidone)藥物具反應之單株抗體。 6. 單株抗體純化及特性分析。預期效益： 1. 完成富來頓(Furazolidone)藥物衍生物的製備各50 mg。 2. 完成富來頓(Furazolidone)衍生物蛋白質結合體的製備藥物-蛋白質結合體各50 mg。 3. 完成動物免疫。 4. 完成ELISA免疫篩選系統之建立。 5. 完成對富來頓(Furazolidone)及代謝物(AOZ)具反應之融合瘤細胞。 6. 完成單株抗體純化及抗體亞型分析。 Specific aims:Development of screening tests for residues of Furazolidone in animal products.Important works in this year: (1) Preparation of derivatives of Furazolidone. (2) Preparation of conjugate complexes of Furazolidone derivatives to different carrier proteins. (3) Immunization of mice for producing monoclonal antibodies. (4) Establishment of ELISA system to screen antiserum and monoclonal antibodies. (5) Cell fusion and screening of monoclonal antibodies.Select monoclonal antibodies against Furazolidone and AOZ. (6) Purification and characterization of monoclonal antibodies.Expected Results: (1) Produce Furazolidone hapten derivatives-conjugates 50 mg. (2) Produce Furazolidone protein-conjugate antigens 50 mg. (3) Animal immunization. (4) Establish specific ELISA system to screen monoclonal antibodies. (5) Produce monoclonal antibodies against Furazolidone. (6) Harvest and characterize pure monoclonal antibodies. 研究期間：9301 ~ 9312
Relation:	財團法人國家實驗研究院科技政策研究與資訊中心
Appears in Collections:	[Department of Chemistry] Research Project

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