完成AOZ酵素免疫檢測試劑雛型開發： 1. AOZ抗體之生產及純化及特性分析確認。 2. AOZ抗原衍生物之製備。 3. AOZ標準品製備。 4. AOZ抗原衍生物-HPP酵素結合體製備。 5. AOZ ELISA試劑雛型建立。 6. 雛形套組品質測試結果及小量樣品測試。預期效益： 1. 完成AOZ抗體之純化50 mg。 2. 完成AOZ抗原衍生物100 mg。 3. 完成AOZ標準品之製備。 4. 完成AOZ抗原衍生物-HPP酵素結合體製備。 5. 建立AOZ ELISA試劑雛型。 6. 完成AOZ ELISA試劑產品品質測試及田間樣品測試。 Specific aims: Development of Competitive ELISA kit for residues of AOZ (Furazolidone metabolism) in animal products. Important works in this year: 1. Production and purification for anti- AOZ antibody. 2. Preparation of derivatives of AOZ (Furazolidone metabolism). 3. Preparation of Standard sample of AOZ (Furazolidone metabolism). 4. Find out proper condition for AOZ derivatives -HRPO conjugation. 5. Establish competitive ELISA assay system. 6. Farm trial in small scale. Expected results: 1. Finish 50mg of purification of anti- AOZ antibody. 2. Finish 100 mg of preparation of derivatives of AOZ (Furazolidone metabolism). 3. Finish 100 mg preparation of Standard sample of AOZ (Furazolidone metabolism). 4. Finish 10 mg of purification of AOZ derivatives -HRPO conjugation. 5. Finish establishment of competitive ELISA model. 6. Finish the farm trial in small scale. 研究期間：9402 ~ 9412
