微晶片被廣泛應用於各方研究，無論是整個基因體表現模式或是其中特定區塊的探討，皆深受研究者的喜愛。我們從國防醫學中心微生物及免疫所取得肝癌組織的甲基化晶片，並由美國國家衛生研究院生物資訊中心 (National Center for Biotechnology Information; NCBI) 提供的資料庫 Gene Expression Omnibus (GEO) 取得相對應C型肝癌的基因表現晶片，分別藉由基因的挑選建立許多基因表單，交叉比對相關性。由結果顯示，從腫瘤周圍的正常組織到腫瘤組織甲基化程度的改變是非常劇烈的，當基因上游發生甲基化，基因下游可能受其他狀況影響，導致甲基化與基因表現沒有一定的結果，從中對照一些已發表的基因甲基化與表現情況加以探討。 There is a tendency towards high throughput technology in many aspects. A large number of researches utilize microarray to study the whole genome or the majority of specific sites that would be concerned. We use the HCC methylation beadchip obtained from the Department of Microbiology and Immunology at National Defense Medical Center, and download the raw data of HCV relative expression array from The Gene Expression Omnibus (GEO) database was established by National Center for Biotechnology Information (NCBI) to compare with methylation array data. Construct gene lists by cross mapping gene selection. We show the evidence that the variation of gene methylation level is obvious between the tumor tissues and peripheral tissues. When the methylation occurs at the upstream of a gene, its downstream may be affected by other factors at the same time. To have a comparison with the condition of some genes were proved by others, this study may provide a point of view to combine epigenetic knowledge and expression data, therefore make if helpful for our further research.
