探討光照強度及波長對於Chlorella sp.生產活性多醣 的影響 ; Effects of light intensity and light wavelength on the production of active polysaccharides by Chlorella sp. in batch cultures

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Title:	探討光照強度及波長對於Chlorella sp.生產活性多醣的影響;Effects of light intensity and light wavelength on the production of active polysaccharides by Chlorella sp. in batch cultures
Authors:	陳昆延;Kun-yan Chen
Contributors:	化學工程與材料工程研究所
Keywords:	光波長;光強度;小球藻;Chlorella sp.;light intensity;light wavelength
Date:	2011-07-19
Issue Date:	2012-01-05 11:21:57 (UTC+8)
Abstract:	小球藻Chlorella sp.因其具有相當高的營養價值且培養容易，所以是一種已商業化生產的微藻。由於能源危機慢慢浮現，可生產生質柴油的Chlorella sp.成為各國研究的重點之一，再加上近年來現代人對抗癌藥物的需求逐漸增加，所以可生產多醣體之Chlorella sp.漸漸受到重視，而Chlorella sp.因其具有葉綠素可捕捉光能，故光照對於Chlorella sp.生長的影響為一相當重要的因素。有鑒於此，本研究將對於光照對Chlorella sp.生長之影響作深入之探討，設計以白光強度1000、2000、4000、6000、8000、10000 Lux時對於Chlorella sp.生長及產生胞內及胞外多醣體之影響；另外由Chlorella sp.的全波掃描圖可發現Chlorella sp.對光波長的主要吸收峰為藍光區及紅光區，故接著以藍光及紅光之LED燈進行單一色光對Chlorella sp.生長之影響。白光實驗結果顯示Chlorella sp.在白光強度6000 Lux以下時，生長速率µ、胞內多醣(IPS)及胞外多醣(EPS)會隨強度增加而提高，而生長速率在4000 Lux時有最大值µ=0.07 hr-1，胞外多醣在6000 Lux時有30.41 mg L-1的多醣產量；胞內多醣則是在強度8000 Lux有52.12 mg L-1的最大產量；色光實驗方面，紅光可促進Chlorella sp.藻體的生長，在強度1000 Lux時有最大的藻體產量0.73 g L-1，而藍光則可以促使Chlorella sp.產生產物，在強度1000 Lux時有最好的胞內多醣產率0.55 mg L-1hr-1。動物生物活性實驗方面，胞內多醣的生物活性較胞外多醣高，而以白光培養所得到之多醣體生物活性高於單一色光培養所得之多醣體，最好的生物活性為白光8000 Lux下培養所得之胞內多醣，其生物活性與螺旋藻的胞內多醣相比大約提升了22 %。 Chlorella sp. has used in the commercialization production because of it has many nutrition and easy to culture. In recent years, because the energy crisis reappears and Chlorella sp. can produce biodiesel, Chlorella sp. becomes the research key point of various countries. In addition, people increased to the demand of anticancer treatment medicine, so the research about Chlorella sp. takes seriously because it can produce activated polysaccharides. Chlorella sp. has the chlorophyll and is possible to absorb the light energy so light is a important factor effecting Chlorella sp. growth. This research had studied illumination influence in Chlorella sp. growth. Experimental used different light intensity, included 1000, 2000, 4000, 6000, 8000, 10000 Lux to culture Chlorella sp. and produce polysaccharides, then used colored light, blue LED and red LED, culture Chlorella sp.. In light intensity experiment, polysaccharides production increased with light intensity raises, Chlorella sp. has the maximum specific growth rate 0.007 hr-1 in 4000 Lux. In polysaccharides production, 6000 Lux has the maximum extracellular polysaccharides 30.41 mg L-1 and 8000 Lux has the maximum intercellular polysaccharides 52.12 mg L-1. In colored light experiment, red light can promote Chlorella sp. growth and blue light promote product production, Both's best light intensity is 1000 Lux. In biological activity experiment, intercellular polysaccharides’s biological activity was better than extracellular polysaccharides. The best biological actived polysaccharides was intercellular polysaccharides culturing under 8000 Lux, which promoted the intercellular polysaccharides activity 22 % than Spirulina intercellular polysaccharides.
Appears in Collections:	[National Central University Department of Chemical & Materials Engineering] Electronic Thesis & Dissertation

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