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    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/49961

    Title: Osteoblast Differentiation of Amniotic Fluid-Derived Stem Cells Irradiated with Visible Light
    Authors: Higuchi,A;Shen,PY;Zhao,JK;Chen,CW;Ling,QD;Chen,H;Wang,HC;Bing,JT;Hsu,ST
    Contributors: 化學工程與材料工程學系
    Date: 2011
    Issue Date: 2012-03-27 16:27:28 (UTC+8)
    Publisher: 國立中央大學
    Abstract: The effect of visible light irradiation on the expression of pluripotent genes (Oct-4, Sox2, and Nanog) in amniotic fluid-derived stem cells (AFSCs) and on the osteogenic differentiation ability of AFSCs was investigated using light-emitting diodes (LEDs) at 0-2mW/cm(2) in various wavelengths : [blue (470 nm), green (525 nm), yellow (600 nm), and red (630 nm)]. Pluripotent gene expression in AFSCs was up-regulated by visible light irradiation from a LED for more than 6 h. Green light irradiation of AFSCs up-regulated the expression of pluripotent genes more significantly than irradiation with other light. The osteogenic differentiation of AFSCs was facilitated by green and blue light irradiation. Facilitated differentiation into osteogenic cells by visible light irradiation was not mediated by reactive oxygen species (ROS); alkaline phosphatase activity (a marker of early osteogenic differentiation) and gene expression of osteopontin (a marker of late osteogenic differentiation) did not change significantly between AFSCs in differentiation medium with or without a ROS scavenger (vitamin C). The mitogen-activated protein kinase/extracellular signal-regulated protein kinase pathway, as well as other unknown signaling pathways, may be responsible for the activation of signaling pathways that facilitate the differentiation of AFSCs into osteogenic cells on light irradiation.
    Appears in Collections:[化學工程與材料工程學系 ] 期刊論文

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