Dioscorea Phytocompounds Enhance Murine Splenocyte Proliferation Ex Vivo and Improve Regeneration of Bone Marrow Cells In Vivo

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Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/50813

Title:	Dioscorea Phytocompounds Enhance Murine Splenocyte Proliferation Ex Vivo and Improve Regeneration of Bone Marrow Cells In Vivo
Authors:	Su,PF;Li,CJ;Hsu,CC;Benson,S;Wang,SY;Aravindaram,K;Chan,SI;Wu,SH;Yang,FL;Huang,WC;Shyur,LF;Yang,NS
Contributors:	生命科學系
Keywords:	TRADITIONAL CHINESE MEDICINE;ECHINACEA-PURPUREA;FED RATS;BACTERIA;MODULATION;DIOSGENIN;EXTRACTS;SPLEEN;MICE;GUT
Date:	2011
Issue Date:	2012-03-27 18:10:35 (UTC+8)
Publisher:	國立中央大學
Abstract:	Specific cytokines have been tested clinically for immunotherapy of cancers; however, cytotoxicity has often impaired their usefulness. Consequently, alternative approaches are increasingly desirable. Dioscorea spp. tuber is a widely used traditional Chinese medicinal herb claimed to confer immunostimulatory activity. In this study, we evaluated Dioscorea as an adjuvant therapy for use alongside chemotherapy for cancer. Phytocompounds from Dioscorea tubers were ethanol fractioned and used for ex vivo splenocyte proliferation assay or in vivo force-feeding of mice pre-treated with the chemotherapy agent 5-fluorouracil. Co-treatment with a 50-75% ethanol-partitioned fraction of the tuber extract of D. batatas (DsCE-II) and interleukin (IL)-2 resulted in a significantly higher rate of murine splenocyte cell proliferation ex vivo than treatment with DsCE-II or IL-2 alone. This DsCE-II fraction, which contains a polysaccharide with a high proportion of beta-1,4-linkage mannose (>= 64%), also promoted the regeneration of specific progenitor cell populations in damaged bone marrow tissues of 5-fluorouracil-treated mice. Colony-forming unit (CFU) analyses demonstrated that the population of CFU-GM cells, but not CFU-GEMM or BFU-E cells, preferentially recovered to similar to 67% in the bone marrow of immune-suppressed mice fed with DsCE-II. DsCE-II efficacy level was similar to 85% of that obtained by subcutaneous administration of recombinant G-CSF proteins (5 mu g kg(-1)) in mice tested in parallel. This study suggests that the DsCE-II fraction of D. batatas extract may be considered for further development as a dietary supplement for use alongside chemotherapy during cancer treatment.
Relation:	EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
Appears in Collections:	[Department of Life Science] journal & Dissertation

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