Rapid and specific influenza virus detection by functionalized magnetic nanoparticles and mass spectrometry

NCU Institutional Repository > 理學院 > 化學學系 > 期刊論文 > Item 987654321/50919

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.lib.ncu.edu.tw/handle/987654321/50919

题名:	Rapid and specific influenza virus detection by functionalized magnetic nanoparticles and mass spectrometry
作者:	Chou,TC;Hsu,W;Wang,CH;Chen,YJ;Fang,JM
贡献者:	化學學系
关键词:	HUMAN PLASMA-PROTEINS;RECEPTOR-BINDING;MULTIPLEXED IMMUNOASSAY;DIAGNOSTIC MICROARRAY;HEMAGGLUTININ;PARTICLES;DIFFERENTIATION;IDENTIFICATION;SURVEILLANCE;QUANTITATION
日期:	2011
上传时间:	2012-03-27 18:12:54 (UTC+8)
出版者:	國立中央大學
摘要:	Background: The timely and accurate diagnosis of specific influenza virus strains is crucial to effective prophylaxis, vaccine preparation and early antiviral therapy. The detection of influenza A viruses is mainly accomplished using polymerase chain reaction (PCR) techniques or antibody-based assays. In conjugation with the immunoassay utilizing monoclonal antibody, mass spectrometry is an alternative to identify proteins derived from a target influenza virus. Taking advantage of the large surface area-to-volume ratio, antibody-conjugated magnetic nanoparticles can act as an effective probe to extract influenza virus for sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and on-bead mass spectrometric analysis. Results: Iron oxide magnetic nanoparticles (MNP) were functionalized with H5N2 viral antibodies targeting the hemagglutinin protein and capped with methoxy-terminated ethylene glycol to suppress nonspecific binding. The antibody-conjugated MNPs possessed a high specificity to H5N2 virus without cross-reactivity with recombinant H5N1 viruses. The unambiguous identification of the captured hemagglutinin on magnetic nanoparticles was realized by SDS-PAGE visualization and peptide sequence identification using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Conclusions: The assay combining efficient magnetic separation and MALDI-MS readout offers a rapid and sensitive method for virus screening. Direct on-MNP detection by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) provided high sensitivity (similar to 10(3) EID(50) per mL) and a timely diagnosis within one hour. The magnetic nanoparticles encapsulated with monoclonal antibodies could be used as a specific probe to distinguish different subtypes of influenza.
關聯:	JOURNAL OF NANOBIOTECHNOLOGY
显示于类别:	[化學學系] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	498	检视/开启

在NCUIR中所有的数据项都受到原著作权保护.

社群 sharing

数据加载中.....