Diagnostic Detection of Human Lung Cancer-Associated Antigen Using a Gold Nanoparticle-Based Electrochemical Immunosensor

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题名:	Diagnostic Detection of Human Lung Cancer-Associated Antigen Using a Gold Nanoparticle-Based Electrochemical Immunosensor
作者:	Ho,JAA;Chang,HC;Shih,NY;Wu,LC;Chang,YF;Chen,CC;Chou,C
贡献者:	光電科學與工程學系
关键词:	NEURON-SPECIFIC ENOLASE;CEREBROSPINAL-FLUID;MASS-SPECTROMETRY;TUMOR-MARKER;HUMAN-SERUM;ASSAY;IDENTIFICATION;ENHANCEMENT;STABILITY;PROTEINS
日期:	2010
上传时间:	2012-03-27 18:14:02 (UTC+8)
出版者:	國立中央大學
摘要:	The development of rapid and sensitive methods for the detection of immunogenic tumor-associated antigen is important not only for understanding their roles in cancer immunology but also for the development of clinical diagnostics. a-Enolase (ENO1), a p48 molecule, is widely distributed in a variety of tissues, whereas gamma-enolase (ENO2) and beta-enolase (ENO3) are found exclusively in neuron/neuroendocrine and muscle tissues, respectively. Because ENO1 has been correlated with small cell lung cancer, nonsmall cell lung cancer, and head and neck cancer, it can be used as a potential diagnostic marker for lung cancer. In this study, we developed a simple, yet novel and sensitive, electrochemical sandwich immunosensor for the detection of ENO1; it operates through physisorption of anti-ENO1 monoclonal antibody on polyethylene glycol-modified disposable screen-printed electrode as the detection platform, with polyclonal secondary anti-ENO1-tagged, gold nanoparticle (AuNP) congregates as electrochemical signal probes. The immunorecognition of the sample ENO1 by the congregated AuNP@antibody occurred on the surface of the electrodes; the electrochemical signal from the bound AuNP congregates was obtained after oxidizing them in 0.1 M HCl at 1.2 V for 120 s, followed by the reduction of AuCl(4-) in square wave voltammetry (SWV) mode. The resulting sigmoidally shaped dose response curves possessed a linear dynamic working range from 10(-8) to 10(-12) g/mL. This AuNP congregate-based assay provides an amplification approach for detecting ENO1 at trace levels, leading to a detection limit as low as 11.9 fg (equivalent to 5 mu L of a 2.38 pg/mL solution).
關聯:	ANALYTICAL CHEMISTRY
显示于类别:	[光電科學與工程學系] 期刊論文

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