English  |  正體中文  |  简体中文  |  Items with full text/Total items : 70585/70585 (100%)
Visitors : 23188770      Online Users : 568
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version


    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/51532


    Title: Urinary CD14 as a potential biomarker for benign prostatic hyperplasia - discovery by combining MALDI-TOF-based biostatistics and ESI-MS/MS-based stable-isotope labeling
    Authors: Cheng,HL;Huang,HJ;Ou,BY;Chow,NH;Chen,YW;Tzai,TS;Wu,CJ;Chen,SH
    Contributors: 通訊工程學系
    Keywords: QUANTITATIVE PROTEOMICS;MASS-SPECTROMETRY;2-DIMENSIONAL ELECTROPHORESIS;LIQUID-CHROMATOGRAPHY;CANCER;MEN;ALPHA-1-ANTICHYMOTRYPSIN;IDENTIFICATION;PROTEINS;DISEASE
    Date: 2011
    Issue Date: 2012-03-27 18:55:25 (UTC+8)
    Publisher: 國立中央大學
    Abstract: Purpose: Quest for specific urinary biomarkers for benign prostatic hyperplasia (BPH). Experimental design: Proteomics studies were conducted with urines of the training set to discovering marker candidates that could differentiate BPH from normal subjects by matching results deduced from MALDI-TOF of individual samples and results deduced from nanoLC-ESI-MS/MS-based stable isotope dimethyl labeling of two pooled samples (BPH and normal). Samples were digested before analysis and such an approach takes into account the subject-to-subject variation and differential amount, as well as protein identification. Selected markers were validated by ELISA conducted on the training set and the test set as well as another set of urines collected from prostate cancer patients. Results: Nine marker candidates were identified from proteomics studies; CD14, prostate-specific antigen and pancreatic a-amylase precursor were further selected for ELISA validation. Urinary CD14 is among the best match with high specificity (>81%) for both training and test sets. In addition, from the study of prostate cancer patients, CD14 also allows the distinction of BPH from cancer with high specificity (84-100%) when combined with urinary prostate-specific antigen. Conclusions and clinical relevance: Urinary CD14 is suggested to have a high specificity in the diagnosis of BPH in distinction from normal as well as cancer subjects.
    Relation: PROTEOMICS CLINICAL APPLICATIONS
    Appears in Collections:[通訊工程學系] 期刊論文

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML750View/Open


    All items in NCUIR are protected by copyright, with all rights reserved.

    社群 sharing

    ::: Copyright National Central University. | 國立中央大學圖書館版權所有 | 收藏本站 | 設為首頁 | 最佳瀏覽畫面: 1024*768 | 建站日期:8-24-2009 :::
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback  - 隱私權政策聲明