光學三角量測方法量測斜紋夜蛾在不同試劑中肌肉振動變化之研究;The Measurement of Muscle Vibration of Spodoptera litura in Different Reagent Concentration Based on Optical Triangulation Method

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题名:	光學三角量測方法量測斜紋夜蛾在不同試劑中肌肉振動變化之研究;The Measurement of Muscle Vibration of Spodoptera litura in Different Reagent Concentration Based on Optical Triangulation Method
作者:	黃志弘;Huang,Chih-hung
贡献者:	光電科學研究所碩士在職專班
关键词:	斜紋夜蛾;三角量測;Triangulation;spodoptera
日期:	2012-08-28
上传时间:	2012-09-11 18:39:35 (UTC+8)
出版者:	國立中央大學
摘要:	本論文中，我們利用光學三角測量法，成功地利用CMOS sensor、雷射光及MATLAB軟體分析等工具，針對我們所選用的斜紋夜蛾動物模式，量測其肌肉收縮變化的型態模式，將肉眼不易辨識的動態變化，以精密度達10 μm，數量化細微的物點位移，分別得到各種不同刺激(空針扎刺、12% 福馬林、37%福馬林) 的光點變動與時間的關係圖以及頻譜圖。以注射方式，以空針扎刺或注射藥劑200nl注射於斜紋夜蛾背部第七體節後，量測其肌肉收縮之變化，並將波形變動轉換成頻率(或頻譜圖)。結果顯示以空針扎刺之物理傷害，並未對斜紋夜蛾幼蟲造成嚴重而持續性傷害；但福馬林處理引起之蟲體肌肉則有相當明顯的變化，但福馬林引起之振幅變化，並未隨著濃度持續增加而變大。顯示適當之刺激，較過量之刺激，更能得到明顯之變化而易於觀察。而溶液之流動方向亦影響振幅之變化。In this paper, we used an optical triangulation method，CMOS sensor, laser light and MATLAB software analysis tools, we selected Spodoptera litura to measure the muscle contraction patterns of change mode to the naked eye hard to identification of dynamic changes to the precision up to 10 μm, the number of minor object point displacement, respectively, to get a variety of stimuli (empty needle stab, 12% formalin, 37% formalin) change in the spot diagram of the time and spectrum. Injecting an empty needle stab inoculation 200nl was injected into the 7th segment of the Spodoptera litura back after the holiday, the measured muscle contraction of the change, and the waveform changes to convert the frequency (or frequency spectrum).The results showed that the empty needle stab of physical injury, serious and persistent damage is not caused by Spodoptera litura larvae; Formalin processing caused by the parasite muscle is quite obvious change, but Formalin-induced amplitude changes did not increase with the concentration continued to increase and become larger.Display the appropriate stimulus, excessive stimulation, the more obvious changes easy to observe. The flow direction of the solution also impact the amplitude change.
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