中大機構典藏-NCU Institutional Repository-提供博碩士論文、考古題、期刊論文、研究計畫等下載:Item 987654321/62356
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 70585/70585 (100%)
造訪人次 : 23041171      線上人數 : 507
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋


    請使用永久網址來引用或連結此文件: http://ir.lib.ncu.edu.tw/handle/987654321/62356


    題名: 利用電場控制導電性高分子以進行基因於聚電解質多層膜的組裝與釋放;The assembly and disassembly of polyelectrolyte multilayer regulated by electrical control using conductive polymer
    作者: 胡威文
    貢獻者: 國立中央大學化學工程與材料工程學系
    關鍵詞: 化學工程;生物技術(醫)
    日期: 2013-12-01
    上傳時間: 2014-03-17 11:30:55 (UTC+8)
    出版者: 行政院國家科學委員會
    摘要: 研究期間:10208~10307;In this study, we would like to develop an electrical control gene delivery using conductive polymer as substrates. DNA will be incorporated with chitosan as polyelectrolyte multilayers (PEMs) using layer-by-layer (LbL) techniques. Because the electrostatic interaction is the main driving force to immobilize polycation and polyanion on surfaces, the polarity of substrates should be able to be controlled through adjusting external electrical fields. The electrical effects on the assembly and disassembly of PEMs will be both studied. Conductive polymer, polypyrrole (PPy) films, will be synthesized using different concentrations of monomer and initiator. Their morphology, thickness, and electrical properties will be analyzed. To regulate DNA assembly, vertical electrical fields will be applied. DNA loading capacities, their protection ability to DNA, and cytotoxicity will be examined to ensure that the potential may significantly improve DNA incorporation without eliciting negatively effects on surface cells. On the other hand, we will also develop an electrical control of PEMs disassembly to regulate DNA release from substrates. Horizontal electrodes will be placed in two ends of PPy films. Different electrical stimulation will be applied to study their effects on the unstablization of PEMs. Finally, we will apply this electrical control system to in situ deliver osteogenic factor gene, BMP-7, to placenta-derived multipotent cells (PDMCs) on PPy films. The biocompatibility and cytotoxicity of this gene delivery system will be assessed by MTT and LDH assays, respectively. The internalized BMP-7 gene will be quantified by quantitative real-time PCR, and the expressed BMP-7 will be examined by ELISA. The time sequence of BMP-7 expression will be determined with the levels of mRNA encoding osteoblast differentiation markers. The mineralization level will also be assessed to determine the effect of DNA immobilization strategy on mineral tissue regeneration. With or without applying electrical field will be performed to distinguish the effects of gene delivery and electrical stimulation on cell differentiation. Through these assays we may determine the extent to which this in situ gene delivery method could temporally control cell differentiation and tissue regeneration in specific period. We believe that this interdisciplinary and perspective project should benefit the field of regenerative gene therapy, expand the depth and extent of the tissue engineering research, and increase the visibility of our country in sciences.
    關聯: 財團法人國家實驗研究院科技政策研究與資訊中心
    顯示於類別:[化學工程與材料工程學系 ] 研究計畫

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html0KbHTML290檢視/開啟


    在NCUIR中所有的資料項目都受到原著作權保護.

    社群 sharing

    ::: Copyright National Central University. | 國立中央大學圖書館版權所有 | 收藏本站 | 設為首頁 | 最佳瀏覽畫面: 1024*768 | 建站日期:8-24-2009 :::
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋  - 隱私權政策聲明