為瞭解植物耐受高溫逆境之機轉,進而以遺傳工程改良作物耐熱力以因應全球暖化之威脅,本實驗室以前向式遺傳學的策略,自ethyl methanesulfonate (EMS)處理過之阿拉伯芥M2子代中,篩選出一對高溫逆境失去耐受性之突變植株,命名為heat-intolerant 4 (hit4)。進一步的生理實驗發現,hit4雖失去先天性熱耐受能力(basal thermotolerance),卻仍保有後天性熱耐受能力(acquired thermotolerance)。經基因定位、互補實驗及序列比對,確認hi;The Arabidopsis mutant heat-intolerant 4 (hit4) was isolated from an ethyl methanesulfonate-mutagenized M2 population on the basis of its inability to withstand prolonged heat stress (four days at 37°C). Further characterization indicated that hit4 was im;研究期間:10408~10507