以螢光染料方法研究脂質微胞的滲漏; Leakage of lipid vesicle studied by dye fluorescence method

Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/7365

Title:	以螢光染料方法研究脂質微胞的滲漏;Leakage of lipid vesicle studied by dye fluorescence method
Authors:	陳夙容;Su-Jung Chen
Contributors:	物理研究所
Keywords:	滲漏;螢光染料方法;脂質微胞;leakage;vesicle;fluorescence method
Date:	2007-07-05
Issue Date:	2009-09-22 10:56:28 (UTC+8)
Publisher:	國立中央大學圖書館
Abstract:	抗菌蛋白的殺菌機制為近年來廣為研究的課題。本實驗以DOPC脂質分子所製備的微胞，內含高濃度螢光染劑Calcein，並以蜂毒蛋白melittin在微胞上打洞造成螢光染劑的滲漏，來研究蜂毒蛋白與脂膜的作用。實驗結果顯示: 滲漏率隨著melittin的濃度而線性增加，當melittin與脂膜分子比率達到6/1000則有100%滲漏。推斷是蜂毒蛋白吸附脂膜時造成瞬間的裂縫而使滲漏率線性增加; 推斷有100%滲漏，是因蜂毒蛋白在膜上形成孔洞所致。 The mechanism of antimicrobial peptide has been extensively studied. In this thesis, we measured the melittin-inducing leakage of calcein from DOPC vesicles to study the interaction between melittin and lipid membrane. The result revealed that the leakage increases in linear with the melittin concentration, and reaches to 100% leakage as the molar ratio of melittin to lipids becomes larger than 6/1000. The linear increase is attributed to the transient disruption upon melittin binding to the membrane, whereas 100% leakage is due to the pore formation induced by high concentration peptides.
Appears in Collections:	[Graduate Institute of Physics] Electronic Thesis & Dissertation

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