結合基板引導膠原蛋白自組裝法與利用紫外光曝光及胰蛋白酶蝕刻產生微米圖案的技術來製作膠原蛋白纖維島嶼以應用於細胞表現型之控制;Control of cell phenotype by collagen fibril islands fabricated by substrate-guided self-assembly of collagen molecules and micro-patterning using masked UV exposure and trypsin etching

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题名:	結合基板引導膠原蛋白自組裝法與利用紫外光曝光及胰蛋白酶蝕刻產生微米圖案的技術來製作膠原蛋白纖維島嶼以應用於細胞表現型之控制;Control of cell phenotype by collagen fibril islands fabricated by substrate-guided self-assembly of collagen molecules and micro-patterning using masked UV exposure and trypsin etching
作者:	邢永年;Xing, Yong-Nian
贡献者:	物理學系
关键词:	膠原蛋白;微米圖案;幹細胞;原子力顯微鏡;Collagen;Micro-patterning;MSC;AFM
日期:	2019-01-29
上传时间:	2019-04-02 14:33:15 (UTC+8)
出版者:	國立中央大學
摘要:	研究顯示，微米及奈米尺度的基板會影響貼附在上面的細胞，包括貼附形狀、遷移、繁殖、分化等等。細胞外基質像膠原蛋白纖維這種具有特殊結構的基板在控制細胞的表現型上面就扮演了重要的角色。在本實驗中，我們結合自組裝膠原蛋白分子和微米圖案光刻製造膠原蛋白纖維島嶼，我們把人類間充質幹細胞(MSC)養在膠原蛋白纖維島嶼基板上面，觀察細胞是否會貼附在島嶼上面並改變自身形狀，並在基板製作時滴入PLL-g-PEG這種聚合物，之後觀察細胞貼附在島嶼上的情形分析PLL-g-PEG對基板的影響，藉由觀察細胞貼附的狀況來改善PLL-g-PEG的參數，最終達到完全控制細胞的貼附形狀。未來，我們會加入不同的分化劑來觀察MSC貼附在不同的膠原蛋白纖維島嶼上的分化速度，再用共厄焦顯微鏡來觀察細胞內的變化，像是用DNA、F-atin等等，利用觀察的結果改變膠原蛋白纖維島嶼的形狀，讓MSC分化的速度到達最快。;It has been known that the microscale and nanoscale substrate topography of cell-culturing substrate can regulate cell adhesion, migration, proliferation, and diﬀerentiation. It is also known that extracellular matrix is made of most importantly collagen ﬁbrils, which possess speciﬁc molecular identity and structural characteristics that may be crucial for controlling cell behaviors such as cell phenotype. In this work, we combined the techniques of self-assembly of collagen molecules and lithographic micropatterning to produce collagen ﬁbril islands. The collagen ﬁbril island substrates were used to culture human mesenchymal stem cells (MSC) to control their shape. We also added PLL-g-PEG on the substrate and observed the cell type to know PLL-g-PEG contribution. In future, We will observe MSC diﬀerentiation rate by various collagen ﬁbril islands.We will use DNA, F-actin to observe cell internal transform by collagen ﬁbril islands.
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