探討光照對Rhizopus oligosporus轉換豆漿中異黃酮醣苷之影響;Effect of light condition on bioconversion of isoflavone glycosides in soymilk by Rhizopus oligosporus

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题名:	探討光照對Rhizopus oligosporus轉換豆漿中異黃酮醣苷之影響;Effect of light condition on bioconversion of isoflavone glycosides in soymilk by Rhizopus oligosporus
作者:	楊佳叡;Yang, Chia-Jui
贡献者:	化學工程與材料工程學系
关键词:	異黃酮;少孢根黴菌;兩階段;大豆苷元;染料木黃酮;光照
日期:	2020-01-15
上传时间:	2020-06-05 17:03:55 (UTC+8)
出版者:	國立中央大學
摘要:	苷元型異黃酮(isoflavone aglycones)因具有降低心血管疾病、預防骨質疏鬆、抗腫瘤、改善肥胖症等特性，因此被廣泛地討論。本實驗利用微生物發酵法，以豆漿取代傳統大豆作為基質，透過Rhizopus oligosporus將醣苷型異黃酮(isoflavone glucosides)轉化成苷元型異黃酮(isoflavone aglycones)。而在微生物發酵中，不同光源也會影響微生物的生理狀態和發酵行為，因此本研究主要的目的是在於探討「光照」環境因子對於Rhizopus oligosporus生產苷元型異黃酮之影響，探討不同光源以及不同光照度對於菌體的生長及產物的變化。本研究結果顯示在光照的條件下，紅光200 lux最有利於Rhizopus oligosporus的菌體生長，能將菌體重量提升至避光組的1.38倍，而在藍光100 lux的情況下，則有最佳的單位菌重轉化產物能力，約為控制組的1.35倍。因此，本研究設計了以紅光接續藍光的兩階段光照的策略，以提升最佳的菌體重量與產物產量。在兩階段光照的條件下，大豆苷元的產量可以高達24.5 µg/ml，與單純紅光照相比及單純藍光照相比，皆提升了約26%。此外，經由兩階段光照條件下的染料木黃酮產量，可提升至20.26 µg/ml，與單純紅光照相比，提升了約15%。 ;Isoflavone aglycones have been reported to help in the prevention of cancer, cardiovascular diseases, osteoporosis, obesity . It plays an important role in human health and has been discussed for several years. Isoflavone aglycones can be produced from isoflavone glucosides. In this work, a fermentation process with soymilk which replaces traditional soybean as substrate has been performed. Besides, different light conditions will also has a strong connection with microbial growth and fermentation behavior. In this work, we have explored the effects of light condition on bioconversion of isoflavone glucosides into isoflavone aglycones using Rhizopus oligosporus as well as develop strategies to enhance isoflavone aglycones production. Experimental results showed that at red light 200 lux condition performed the optimum cells growth, which was 38% higher than dark group. But at blue light condition, which light intensity was 100 lux, was more favorable for product yield based on biomass. It could perform 35% higher than the dark group. Therefore, two-stage shake flask fermentation by light control was conducted. Daidzein concentration using two-stage fermentation was 24.5µg/ml which corresponds to 26 % higher than single-light fermentation at red and blue light. On the other hand, Genisrein concentration using two-stage fermentation was 20.26µg/ml which corresponds to 15 % higher than single-light fermentation at red light.
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