金屬有機骨架材料 (MOFs) 因具規則排列的孔洞，而成為目前最新穎的材料之一，主要特點包括高的熱及化學穩定性、尺寸大小可調控性和孔洞之選擇性。已有報導將金屬有機骨架材料塗層於酵母菌細胞表面，藉此抵禦生物裂解酶的攻擊，並使小分子營養乳糖從孔洞擴散進去而提供細胞養分，然而其表面塗層有缺陷，亦可能使抗生素氨苄青黴素等小分子擴散進去而降解細胞。本研究先合成奈米級類沸石咪唑骨架材料-8 (ZIF-8) 塗層於大腸桿菌表面，並證實抗生素確實可能經由缺陷擴散進去細胞，而使細胞裂解死亡。並進一步利用本實驗室於2015年發表之合成方法，利用微米級類沸石咪唑骨架材料-90 (ZIF-90) 封裝大腸桿菌，使其具備更完整的保護以抵禦抗生素，將材料崩解過後，大腸桿菌仍然可以回到原本的生長狀態。並分別量測大腸桿菌和兩種材料ZIF-8/-90之界達電位，藉此證明界達電位之差值確實會影響形成材料之大小，提供未來合成生物複合材料之參考。;Metal Organic Frameworks (MOFs), an emerging class of porous materials, have become promising materials because of their high thermal and chemical stability, tunable of their pore shape, and size selectivity. There is a report about MOF materials coating on yeast cells to prevent larger cytotoxic molecules, such as lytic enzymes while permit the transport of nutrients necessary for cell viability. However, the defects on MOF-coating may have a chance to enable antibiotic Ampicillin diffuse through and kill the cell. In this study, we demonstrate how to synthesize ZIF-8 coated E. coli and vertify antibiotics could indeed diffuse through and cause E. coli lysis. Herein, we based on our previous report of de novo approach to encapsulate Escherichia coli (E. coli) into ZIF-90 single crystals, E. coli@ZIF-90, as a completely protective structure against harsh conditions. After decomposition of ZIF-90 materials, E. coli immediately regained full functionality. Moreover, we measured the zeta potential of E. coli and ZIF-8/-90 to prove that surface electrostatic potential indeed have a influence on the size of biocomposites.
