本研究利用掃描式白光干涉顯微技術，搭配快速傅立葉轉換法(Fast Fourier Transform, FFT)，將時域干涉訊號轉換為頻域資訊，取得二維空間影像之相位頻譜，應用於侷域表面電漿共振效應(Localized Surface Plasmon Resonance, LSPR)感測器之開發，藉由量測相位共振波長，來避免傳統直接量測強度時易受到的環境干擾。 相位頻譜之金奈米粒子，以金奈米粒子的LSPR吸收光譜之特徵峰值位置的共振波長作為識別；LSPR相位分布強度機率圖則用來確認金奈米粒子相位出現機率與各濃度之間差異性，並引入質心演算法(Spectral Centroid Algorithm)來量化此現象。 藉由觀察LSPR 特徵峰值來分析不同濃度之間的變化，所量測濃度之檢測極限(Limit of Detection, LOD)為0.1485%，意即本系統可以偵測到 〖6.5955×10〗^10 〖mL〗^(-1) 個金奈米粒子。這證實了本研究內所開發的白光干涉顯微成像系統之創新技術可用於量測LSPR感測器相位頻譜之開發，實現大範圍且快速量測分析之檢測平台。期望未來可將本研究應用在發展一套精準、可靠、高靈敏度的多重偵測平台，並實現多重偵測之光學感測技術。;In this study, scanning white light interference microscopy with Fast Fourier Transform (FFT) which can convert the time domain interference signal into frequency domain information and obtain the phase spectrum of the two-dimensional spatial image, was applied to the development of a localized surface plasmon resonance (LSPR) sensor. By measuring the phase resonance wavelength, the environmental interference that is susceptible to traditional direct measurement of intensity can be avoided. The resonance wavelength of the characteristic peak position of the LSPR absorption spectrum of gold nanoparticles is used as the identification and analysis of the phase spectrum. The LSPR phase distribution intensity probability map was used to confirm the phase repeatability and the difference between concentrations of the gold nanoparticles. A spectral centroid algorithm was introduced to quantify this phenomenon. By observing the characteristic peaks of LSPR to analyze the change between different concentrations, the limit of detection (LOD) of the measured concentration is 0.1485%, which means that 〖6.5955×10〗^10 〖mL〗^(-1) gold nanoparticles can be detected by the system. This result confirms that the innovative technique of the white light interference microscopy imaging system developed in this study can be used to measure the phase spectrum of the LSPR sensor, and also provide a detection platform with high sensitivity and fast speed. It is expected that this research can be applied to develop a set of accurate, reliable and high-sensitivity multiple detection platforms in the future and realize the optical sensing technology of multiple detection.
