近年來,數位全像顯微技術(Digital Holographic Microscope,簡稱DHM)蓬勃的發展,具有高速相位成像、定量相位成像以及無損畫質之數位對焦等優勢,但目前市售之全像顯微鏡體積都過於龐大且昂貴,為此本論文提出使用體積全像光導元件結合數位全像顯微鏡,組成體積全像無透鏡數位全像顯微鏡(Volume Holographic Lensless Digital Holographic Microscope, VH-LDHM),並利用實驗成功的驗證本架構能成功的將傳統之傅氏轉換數位全像顯微鏡(Fourier Transform Digital Holographic Microscope, LFT-DHM)縮小化,其具有可微型化、高NA和光學變焦等優勢,使其擺脫昂貴光學顯微鏡與龐大體積的限制,以實現數位全像顯微鏡之可攜帶性。並且在縮小化之架構下,可以不改變物距,仍擁有可切換顯微鏡之放大率的功能,能夠在不同之視野範圍(Field of View,簡稱FOV)下,獲得互相對應的解析度(Resolution)。;In recent years, digital holographic microscopy has developed vigorously. It obtains the advantages of high-speed phase imaging, quantitative phase imaging and digital focusing without losing image quality. However, current commercially available holographic microscopes are too bulky and expensive. Therefore, this thesis proposed a volume holographic optical waveguide element combined with a digital holographic microscope to form a volume holographic lens-less digital holographic microscope. In the experiment, we successfully minimized the Lens-less Fourier Transform Digital Holographic Microscope which has the advantages of high NA, minimizable body and optical zoom. Instead of a heavy and expensive optical microscope, it can realize the portability of a digital holographic microscope. Furthermore, under such a minimized body, the ability of switching magnification of the microscope can be maintained without changing the object distance. In different fields of view, we can obtain the corresponding resolution.