建立三維列印仿生皮膚模型用於經皮藥物測試平台;To Develop a Three-dimensional Printed Biomimetic Skin Model for the Testing Platform of Transdermal Drugs

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題名:	建立三維列印仿生皮膚模型用於經皮藥物測試平台;To Develop a Three-dimensional Printed Biomimetic Skin Model for the Testing Platform of Transdermal Drugs
作者:	洪綺鴻;Hong, Hong-Qi
貢獻者:	生物醫學工程研究所
關鍵詞:	⽪膚三維培養;3R 動物替代;氣液介⾯;⽣物 3D 列印機;3D skin culture;3R animal replacement;Air-liquid interface;3D bio-printing
日期:	2023-02-02
上傳時間:	2023-05-09 17:24:50 (UTC+8)
出版者:	國立中央大學
摘要:	每⼀年，動物實驗的消耗數量約為⼀億五千萬隻，在研究上的花費及動物與⼈體內環境差異甚⾄是社會的道德標準都是⼀項需要被關注及解決的問題。近年來，由於歐盟意識的抬頭，許多國家已經禁⽌經由動物實驗的化妝產品進⼝及設⽴相關法規，但在⽇常使⽤的保養品或是化妝品若無經過⽪膚試驗，恐對我們⼈體造成不可預知的傷害。本研究提出⼀個模仿⼈體⽪膚真實環境來進⾏產品的試驗，解決動物實驗相關限制的問題，並且解決動物與⼈體環境差異問題，模仿⼈體真實⽪膚的部分利⽤來源為動物 I 型去端肽的膠原蛋⽩⽣物墨⽔（Collgel®）作為細胞⽀架並加⼊⼈類成纖維母細胞（Fibroblast, HDF )並使⽤⽣物型 3D 列印機進⾏列印建⽴真⽪層（Dermis）的架構，並於膠原蛋⽩⽣物墨⽔上⽅加⼊⼈類⾓質形成細胞（Keratinocyte, HaCaT）作為表⽪層的替代，以 24 孔盤插⼊式培養⽫（Transwell）進⾏共培養以及形成氣液介⾯，並於 28 天的培養以促使⾓質形成細胞可以有效分化形成具疏⽔性的⾓質層以模擬真實⼈體⽪膚中的特性、⽪膚吸收之情形，將此仿⽣⽪膚平台⽤於化妝品測試並觀察表⽪層通透與否及吸收之情形。;Every year, the consumption of animal experiments is about 150 million. The cost of research, the differences in the internal environment of animals and humans, and even the moral standards of society are issues that need to be paid attention to and solved.In recent years, due to the rising awareness of the European Union, many countries have banned the import of cosmetic products that have undergone animal experiments and established relevant regulations. However, if the skin care products or cosmetics used in daily use are not tested on the skin, they may cause unpredictable harm to our human body.This study proposes a product test that imitates the real environment of human skin, solves the problems related to animal experiments, and solves the problem of differences between animals and human environments. The part that mimics the real skin of the human body uses collagen derived from pig tendon type I atelopeptide. Protein bio-ink (Collgel®) is used as a cell scaffold and human fibroblasts (Fibroblast, HDF ) and printed using a bio-type 3D printer to build the structure of the dermis (Dermis), and added on top of the collagen bio-ink Human keratinocytes (Keratinocyte, HaCaT), as a substitute for the epidermis, were co-cultured in a 24-well insert culture dish (Transwell) and formed an air-liquid interface, and cultured for 28 days to promote the effective differentiation of keratinocytes to form Hydrophobic stratum corneum to simulate the characteristics of real human skin and the situation of skin absorption. Use this bionic skin platform for cosmetic testing and observe whether the epidermis is transparent or not and the situation of absorption
顯示於類別:	[生物醫學工程研究所 ] 博碩士論文

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