循環腫瘤細胞（CTCs）作為液體活檢的可靠生物標誌物顯示出顯著的潛力，提供了一種微創的方法來檢測癌症並監測治療進展。然而，循環腫瘤細胞的分離和檢測仍然是一個主要挑戰。本論文將提出一種利用微通道集成表面增強拉曼散射（SERS）基板來分離和檢測癌細胞的新方法。使用微流體器件中的微柱和不同微通道內部間隙的基於大小的方法來從全血中分離癌細胞。HT-29細胞系被加入血樣中以觀察癌細胞的分離。微通道中的微流體濾波器能夠根據癌細胞與血細胞之間的大小差異從血液中分離出癌細胞。然而，微柱在微通道中導致血液顆粒與血漿分離的流動導致微通道堵塞。使用數值和實驗方法研究了微通道中微柱直徑、形狀和間隙等不同參數對血流中堵塞現象的影響。採用電化學無電鍍方法開發的固態SERS基板用於檢測細胞。集成在微通道中的SERS基板將為快速而敏感地檢測癌細胞提供可能，以用於監測癌症進展和研究對治療的反應。;Circulating tumor cells (CTCs) shows significant promise as reliable biomarkers through liquid biopsies, offering a minimally invasive approach for detecting cancer and monitoring therapeutic progress. However, isolation and detection of circulating tumor cells remains one of the major challenge. This thesis will present a novel method to isolate and detect cancer cells using a microchannel integrated with SERS substrate. A size-based isolation of cancer cells from whole blood using micro pillars and varying gap spacing within the microchannel of microfluidic device is developed. HT-29 cell line was spiked into the blood sample to observe isolation of cancer cells. The microfluidic filters in microchannel was able to isolate cancer cells from blood based on the size difference between cancer cells and blood cells. However, the flow of blood in microchannel with micropillar cause the separation of blood particles from the blood plasma resulting in clogging of the microchannel. The effect of different parameters such as diameter, shape and gap between micropillar in clogging phenomenon during the blood flow in the microchannel was studied using numerical and experimental method. Solid state SERS substrates developed by electroless droplet deposition method was used for detection of the cells. The SERS substrate integrated with the microchannel will provide a fast and sensitive detection of cancer cells for monitoring of cancer progression and studying the response to treatment.
