聚胜肽電解質材料合成及其性質研究分析;Synthesis and Analysis of Polypeptide Based Polyelectrolyte Complex

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Title:	聚胜肽電解質材料合成及其性質研究分析;Synthesis and Analysis of Polypeptide Based Polyelectrolyte Complex
Authors:	何敏瑄;Ho, Min-Hsuan
Contributors:	化學工程與材料工程學系
Keywords:	聚電解質;聚胜肽凝聚複合體;二級結構;polyelectrolyte complex;polypeptide complex;secondary structure
Date:	2024-07-22
Issue Date:	2024-10-09 15:23:20 (UTC+8)
Publisher:	國立中央大學
Abstract:	目前已有多項研究表明，聚陽離子和聚陰離子形成的聚電解質是很有發展性的一種膠囊技術，可用藥物釋放、製藥、食品、紡織等產業。於是本研究針陰陽離子進行配對，研究其混合後之型態組成及物理特性。在實驗中，我們使用不同聚合度的聚賴胺酸 (PLL50、PLL100) 作為聚陽離子；聚麩胺酸 (PLGA100) 、聚天門冬胺酸 (PASP90、PASP140、PASP185、PASP250) 作為聚陰離子。將等電荷濃度的聚陽離子和聚陰離子在緩衝溶液中，透過靜電引力及氫鍵形成聚電解質複合體。在顯微鏡觀察下，聚賴胺酸和聚麩胺酸形成的複合體具有固體沉澱結構，而聚賴胺酸和聚天門冬胺酸形成的複合體則具有凝聚層結構。由FTIR及CD分析中，這兩種結構皆分別展現β-sheet和random coil的二級結構。在材料分析方面，發現凝聚複合體具有較弱的氫鍵，並表現出較高的親水性和水合能力。高水合特性也使其將來有機會應用於不沾黏塗層之開發。 ;Several studies have shown that polyelectrolyte complex can be a promising encapsulation technique and employed in pharmaceutical, drug delivery, food, and textile industries. Here, we focused on the synthesis and analysis the properties of the polyelectrolyte complex. In the experiment, we used poly-L-lysine (PLL50, PLL100) as polycations and poly-L-glutamic acid (PLGA100), poly-aspartic acid (PASP90, PASP140, PASP185, PASP250) as polyanions with different degrees of polymerization. Polycations and polyanions of equal charge concentration were mixed in a buffered solution to form polyelectrolyte complexes via electrostatic attraction and hydrogen bonding. Under microscopic observation, the complexes formed by poly-L-lysine and poly-L-glutamic acid exhibited solid precipitates, while those formed by poly-L-lysine and poly-aspartic acid displayed PCs structure. In FTIR and CD analysis, two products exhibited secondary structures of β-sheet and random coil, respectively. After deposition on culture plates, thin film analysis showed that the PCs had weaker hydrogen bonds, higher hydrophilicity and hydration. This property also make it a candidate of non-fouling material for anti-fouling treatment.
Appears in Collections:	[National Central University Department of Chemical & Materials Engineering] Electronic Thesis & Dissertation

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