應用STARR-Seq技術研究由先驅因子Zelda介導的基因組重編程機制;Investigating Genomic Reprogramming Mechanisms Mediated by the Pioneer Factor Zelda through Starr-Seq

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Title:	應用STARR-Seq技術研究由先驅因子Zelda介導的基因組重編程機制;Investigating Genomic Reprogramming Mechanisms Mediated by the Pioneer Factor Zelda through Starr-Seq
Authors:	粘仲毅
Contributors:	國立中央大學生命科學系
Keywords:	先驅因子;基因組重編程;幹細胞;合子基因激活;基因網絡;轉錄組;增強子;;zygotic genome activation;genomic reprogramming;pioneer factors;baculovirus expression system;STARR-seq;chromatin accessibility;enhancers;Zelda;transcriptome;gene network
Date:	2025-07-31
Issue Date:	2025-08-07 16:54:06 (UTC+8)
Publisher:	國家科學及技術委員會(本會)
Abstract:	This study aims to modify and apply STARR-seq (self-transcribing active regulatory region sequencing) to quantitatively map whole-genome enhancer activities in the present or absent of Zelda. With integrative analysis and meta-analysis of pre-established related datasets, we will be able to discover the bona fide signatures of ZGA enhancer networks, and reconstruct the regulome of ZGA. I have successfully performed NGS-based functional genomic approaches combined with big data analysis (including RNA-seq, transcription factor ChIP-seq, pol II ChIP-seq, MNase-seq), and have published papers in journals with high impact factors. In addition, I have the supports from and communications with the expertise on the approaches used in my aims, including Alexander Stark at IMP, Vienna. I have also finished the most onerous and rate-limiting step which is to generate genomic library for STARR-seq. There, I would expect to accomplish this work. Aim 1. To delineate the chromatin accessibility landscape of Zelda expressing S2 cells. Aim 2. To map quantitative genome-wide Zelda-dependent enhancers activity in S2 cells by STARR-seq. Aim3. To reconstruct the regulome in S2 cells reprogramed by Zelda.
Relation:	財團法人國家實驗研究院科技政策研究與資訊中心
Appears in Collections:	[Department of Life Science] Research Project

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