| 摘要: | 表面增強拉曼散射(Surface-Enhanced Raman Scattering, SERS)技術因其具備超高靈敏度、分子指紋特異性與非破壞性等優點，在近年廣泛應用於生物標誌物檢測、環境污染物監控與藥物殘留分析等多領域。其中，SERS基材的品質決定訊號增強效率與可重現性，然而目前常見的SERS基材製程在熱點密度均勻性、背景雜訊抑制及大面積製備上的穩定性仍面臨挑戰，限制其在定量分析與實際臨床應用上的推展。
本研究以開發高效能、高穩定性的SERS活性基材為核心，提出一種基於液-液界面誘導自組裝技術之製備策略。透過將金屬奈米粒子分散於水相，並與不混溶的有機相形成界面，可於大面積範圍內誘導奈米粒子緊密排列、形成具高度有序性的奈米結構薄膜。此自組裝薄膜可進一步轉移至經疏水改質處理的固體基材上，藉此穩定並提升其表面貼附性與拉曼訊號均勻度。為進一步提升增強效果與訊號穩定性，本研究選用具粗糙分層結構之銀介觀晶體作為SERS活性奈米結構，並搭配碘化鉀處理與氧電漿改質以有效去除表面殘留配體與雜訊源。所製得之銀介觀晶體SERS基材經羅丹明6G（R6G）進行性能測試後，顯示出極佳的靈敏度、明顯訊號放大效果與良好線性響應，驗證本策略於熱點密度控制與基材品質穩定化上的有效性。
為驗證該基材於實際生物醫學應用之可行性，本研究進一步以腦神經傳導物質為模型標的進行分析。神經傳導物質為中樞與周邊神經系統中傳遞訊號之關鍵化學分子，其濃度變化與多種神經退化性疾病及精神疾患密切相關。實驗結果顯示，本SERS基材可有效進行其高靈敏定量偵測，具潛力應用於未來神經疾病早期診斷與監測工具之開發。
綜上所述，本研究成功建立一套簡便可控之SERS基材製備流程，具備高度重現性、結構穩定性與低背景雜訊優勢，為建構高性能拉曼感測平台奠定基礎，並展現其廣泛的應用潛力。
;Surface-Enhanced Raman Scattering (SERS) is a powerful spectroscopic technique known for its ultra-high sensitivity, molecular fingerprint specificity, and non-destructive nature. It has found broad applications in biosensing, environmental monitoring, and pharmaceutical analysis. Among the critical factors that determine the performance of SERS is the quality of the substrate, which directly affects the signal enhancement efficiency and reproducibility. However, challenges remain in achieving uniform hotspot distribution, suppressing background noise, and producing large-area, stable substrates—factors that currently limit the broader adoption of SERS in quantitative analysis and clinical applications.
This study focuses on the development of a high-performance and stable SERS-active substrate based on an interfacial-induced self-assembly strategy. By dispersing metallic nanoparticles in the aqueous phase and introducing a non-miscible organic phase, highly ordered nanoparticle films were formed at the liquid–liquid interface over a large area. These films were successfully transferred onto hydrophobically modified solid substrates, enhancing their structural integrity and signal uniformity. To further improve signal enhancement and reduce background interference, silver mesocrystals with a multi-level rough surface structure were employed. Additionally, potassium iodide treatment and oxygen plasma modification were applied to remove residual organic ligands, effectively lowering background signals. Performance evaluation using Rhodamine 6G (R6G) as a model analyte demonstrated excellent signal enhancement, high sensitivity, and good linear response, validating the effectiveness of the proposed fabrication method in controlling hotspot density and improving substrate stability.
To evaluate the biomedical applicability of the developed substrate, neurotransmitters were selected as model targets. These small molecules are key chemical messengers in the central and peripheral nervous systems, and their concentration fluctuations are closely associated with various neurodegenerative and psychiatric disorders, such as Parkinson’s disease, Alzheimer’s disease, and depression. Experimental results confirmed that the SERS substrate enables highly sensitive and quantitative detection of neurotransmitters, indicating its potential for future use in early diagnosis and monitoring of neurological diseases.
In summary, this work establishes fuck a simple and reproducible fabrication process for SERS substrates featuring high hotspot density, structural stability, and low background interference. The proposed platform not only provides a solid foundation for constructing high-performance Raman sensors but also demonstrates significant potential for biomedical sensing applications. |
