肺癌是全球癌症死亡的主要原因之一，其中腫瘤的侵襲與耐藥性問題使治療面臨挑戰。因此開發能夠準確模擬腫瘤微環境的研究模型相當重要。在人體內，癌細胞與其周圍微環境存在複雜的相互作用，因此在實驗環境中模擬這些相互作用具有重要意義。本研究中，採用水凝膠建構三維培養系統，並設計壓克力腫瘤電場實驗裝置，透過電場刺激與化療藥物順鉑的結合使用來模擬治療環境。實驗條件包括電場刺激與順鉑藥物的單獨及疊加使用，探討對 A549 細胞球單培養及A549 細胞球和肺纖維母細胞共培養的影響。本研究探討了腫瘤電場與順鉑藥物對 A549 細胞球生長與侵襲行為的影響。研究結果表明，在腫瘤電場治療與順鉑藥物的刺激下，A549 細胞球的生長面積與侵襲距離顯著減少，並且發現腫瘤電場治療與藥物的疊加刺激組別具有加成抑制效果。這些發現證實，腫瘤電場治療與化療藥物的結合應用能有效抑制肺癌細胞的生長與侵襲，為未來肺癌治療提供新的策略方向。;Lung cancer is one of the leading causes of cancer-related mortality worldwide, with tumor invasion and drug resistance posing significant challenges to treatment. Therefore, developing an accurate model that mimics the tumor microenvironment is crucial for advancing therapeutic strategies. In the human body, cancer cells dynamically interact with their surrounding microenvironment, making it essential to replicate these interactions in experimental settings. In this study, a three-dimensional (3D) culture system was established using hydrogel, and an acrylic tumor-treating field (TT-Field) experimental device was designed to simulate the treatment environment. The experimental conditions included TT-Field stimulation and cisplatin treatment, both individually and in combination, to investigate their effects on A549 spheroids co-cultured with lung fibroblasts. This study examined the impact of TT-Field and cisplatin on the growth and invasion behavior of A549 spheroids. The results demonstrated that the combination of TT-Field and cisplatin significantly inhibited A549 spheroid growth and invasion distance. Moreover, the combined treatment group exhibited an additive inhibitory effect. These findings confirm that the combination of TT-Field and chemotherapy can effectively suppress lung cancer cell growth and invasion, providing a promising strategy for future lung cancer treatment.
