甘蔗渣(bagasse)是最常見且產量最大的纖維素類農業副產物之一，為糖業 壓榨後剩餘的木質纖維性殘渣。儘管多數甘蔗渣僅作為鍋爐燃料使用，但其豐富 的半纖維素含量使其成為極具潛力的可再生碳源。本研究以永續發展目標第 12 項「責任消費與生產」為指引，建立一套將甘蔗渣資源化的廢棄物加值利用系統。 透過酸水解處理甘蔗渣以回收 D-木糖，進一步結合菸鹼醯胺(NAM)進行酵素催 化反應，實現一鍋式酵素級聯合成菸鹼醯胺腺嘌呤二核苷酸(NADH)。 本系統整合11種經重組表現酵素，並結合聚磷酸鹽驅動之ATP再生機制， 有效推進反應進行，同時避免中間產物純化與多餘廢棄物產生。最終NADH濃 度可達2.7 mM(~1.8 g/L)，轉化效率高達85%。此外，唯一副產物為正磷酸鹽， 可由微藻轉化為polyP進行循環利用，展示本系統良好的綠色化學與資源循環潛 力。 綜合而言，本研究展示了一個以農業廢棄物甘蔗渣為原料、具有經濟效益與 環保潛力的NADH生產平台，為推動綠色經濟與永續生產提供具體實證與應用 潛力。;Bagasse is one of the most common and abundant cellulose-based agricultural residues, representing the fibrous lignocellulosic byproduct remaining after sugarcane juice extraction. Although bagasse is mostly used as boiler fuel, its rich hemicellulose content makes it a promising renewable carbon source. In alignment with Sustainable Development Goal 12: Responsible Consumption and Production, this study establishes the waste valorization platform to convert bagasse into valuable biochemicals. Through acid hydrolysis, D-xylose was recovered from bagasse, and was subsequently reacted with nicotinamide (NAM) via an enzyme-catalyzed process to achieve one-pot cascade synthesis of nicotinamide adenine dinucleotide (NADH). The system integrates 11 recombinant enzymes and incorporates a polyphosphate driven ATP regeneration mechanism, enabling efficient NADH biosynthesis while avoiding intermediate purification and excess waste generation. The final NADH concentration reached 2.7 mM (~1.8 g/L) with a conversion efficiency of up to 85%. Furthermore, the only byproduct, inorganic phosphate, can be recycled by microalgae into polyphosphate (polyP), demonstrating the system’s potential in green chemistry and resource circularity. In summary, this study presents an environmentally and economically viable NADH production platform using agricultural waste bagasse, offering a practical solution for promoting green economy and sustainable production.
