| 摘要: | 多氯乙烯（Trichloroethene, TCE）是常見工業溶劑，因高密度與疏水性易在地下水中累積並造成長期污染，對環境與健康構成嚴重威脅。雖然厭氧脫氯菌（如 Dehalococcoides mccartyi）能將 TCE 還原為無毒乙烯，但現地化學還原過程常產生乙炔副產物，乙炔除具二次污染風險外，更為脫氯酶的競爭性抑制劑，導致整治效率顯著下降；同時，污染場址普遍存在養分匱乏問題，限制生物整治的持續性與穩定性。本計畫提出以嗜乙炔菌 SFB93 與脫鹵球菌共培養之創新策略，SFB93 能將乙炔轉化為醋酸與氫氣並具固氮功能，兼具移除乙炔抑制、供應碳氮源與維持脫氯菌增殖的優勢，展現突破性的代謝互補效應。藉由此共培養系統設計，可有效克服乙炔累積與營養不足兩大瓶頸，提升 TCE 生物整治效率，並降低外部資源依賴與碳排放，展現高適應性、低碳永續與系統韌性的整體價值。預期成果包括發展具應用潛力之創新工法，建立低成本、環境友善的 TCE 去除策略，並提供污染減量與碳效益的量化指標，作為模場試驗與政策推動依據，最終將強化我國污染場址復育技術，促進土壤及地下水環境永續治理，並呼應國家低碳與綠色轉型目標。 ;Trichloroethene (TCE) is a common industrial solvent that readily accumulates in groundwater due to its high density and hydrophobicity, posing serious threats to human health and the environment. Although anaerobic dechlorinators such as Dehalococcoides mccartyi can transform TCE stepwise into non-toxic ethene, in situ chemical reduction often generates acetylene byproducts. Acetylene not only presents secondary pollution risks but also acts as a competitive inhibitor of reductive dehalogenases, significantly impairing dechlorination efficiency. Moreover, nutrient limitation is frequently observed at contaminated sites, further constraining the sustainability and stability of bioremediation. This project proposes an innovative co-culture strategy integrating the acetylene-utilizing bacterium SFB93 with Dehalococcoides. SFB93 metabolizes acetylene via acetylene hydratase to produce acetate and hydrogen and possesses nitrogen-fixing capability, thereby simultaneously removing acetylene inhibition, supplying carbon and nitrogen sources, and sustaining the growth of dechlorinators. This metabolic complementarity enables the co-culture system to overcome both acetylene accumulation and nutrient deficiency, enhancing TCE bioremediation performance while reducing external resource dependence and carbon emissions. The project emphasizes high adaptability to complex hydrogeological conditions, low-carbon and sustainable advantages through microbial metabolic synergy, and system resilience with integrated assessment of pollution reduction, resource cycling, and environmental benefits. The anticipated outcomes include the development of a cost-effective and environmentally friendly TCE removal strategy, the establishment of quantitative indicators for pollution reduction and carbon efficiency to guide pilot-scale applications and policy promotion, and the advancement of academic publications, patents, and talent cultivation. Ultimately, this work will strengthen Taiwan’s capacity for contaminated site restoration, promote sustainable soil and groundwater management, and align with national goals for low-carbon development and green transition. |
