Divergent Alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora descended from a common ancestor through whole-genome duplication followed by asymmetric evolution

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Title:	Divergent Alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora descended from a common ancestor through whole-genome duplication followed by asymmetric evolution
Authors:	李宜學;Chang, Chia-Pei;Chang, Chih-Yao;Lee, Yi-Hsueh;Lin, Yeong-Shin;Wang, Chien-Chia
Contributors:	文學院中國文學系
Keywords:	alanine-tRNA ligase;Alanine-tRNA Ligase - analysis;Alanine-tRNA Ligase - genetics;Amino Acid Sequence;ancestry;Base Sequence;Evolution, Molecular;Gene Duplication;Gene Expression Regulation, Fungal;genes;Genes, Fungal;mitochondria;Molecular Sequence Data;Phylogeny;Saccharomycetales - chemistry;Saccharomycetales - cytology;Saccharomycetales - enzymology;Saccharomycetales - genetics;yeasts
Date:	2015-01-01
Issue Date:	2026-04-21 13:43:48 (UTC+8)
Publisher:	American Society for Microbiology;United States: Taylor & Francis
Abstract:	摘要： Cytoplasmic and mitochondrial forms of a eukaryotic aminoacyl-tRNA synthetase (aaRS) are generally encoded by two distinct nuclear genes, one of eukaryotic origin and the other of mitochondrial origin. However, in most known yeasts, only the mitochondrial-origin alanyl-tRNA synthetase (AlaRS) gene is retained and plays a dual-functional role. Here, we present a novel scenario of AlaRS evolution in the yeast Vanderwaltozyma polyspora . V. polyspora possesses two significantly diverged AlaRS gene homologues, one encoding the cytoplasmic form and the other its mitochondrial counterpart. Clever selection of transcription and translation initiation sites enables the two isoforms to be localized and thus functional in their respective cellular compartments. However, the two isoforms can also be stably expressed and function in the reciprocal compartments by insertion or removal of a mitochondrial targeting signal. Synteny and phylogeny analyses revealed that the AlaRS homologues of V. polyspora arose from a dual-functional common ancestor through whole-genome duplication (WGD). Moreover, the mitochondrial form had higher synonymous (1.6-fold) and nonsynonymous (2.8-fold) substitution rates than did its cytoplasmic counterpart, presumably due to a lesser constraint imposed on components of the mitochondrial translational apparatus. Our study suggests that asymmetric evolution confers the divergence between the AlaRS paralogues of V. polyspora . 其他題名： Mol Cell Biol 出版者： United States: Taylor & Francis 出版日期： 2015-07-01 出處： Molecular and cellular biology, 2015-07, Vol.35 (13), p.2242-2253 版權： Copyright © 2015, American Society for Microbiology 2015 版權： Copyright © 2015, American Society for Microbiology. All Rights Reserved. 版權： Copyright © 2015, American Society for Microbiology. All Rights Reserved. 2015 American Society for Microbiology 識別號： ISSN: 1098-5549 識別號： ISSN: 0270-7306 識別號： ISSN: 1067-8824 識別號： EISSN: 1098-5549 識別號： DOI: 10.1128/MCB.00018-15 識別號： PMID: 25896914
Appears in Collections:	[Department of Chinese Literature] journal & Dissertation

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