BACILLUS AMYLOLIQUEFACIENS生長在高GABA含量稻米刺激膠原蛋白合成以及減緩磷酸三鈣誘導產生的皮膚搔癢

以作者查詢圖書館館藏

、以作者查詢臺灣博碩士

、以作者查詢全國書目

、勘誤回報

、線上人數：12

、訪客IP：3.144.91.201

姓名

Enkhtsetseg Tserenkhuu(Enkhtsetseg Tserenkhuu) 查詢紙本館藏

畢業系所

生物醫學工程研究所

論文名稱

BACILLUS AMYLOLIQUEFACIENS生長在高GABA含量稻米刺激膠原蛋白合成以及減緩磷酸三鈣誘導產生的皮膚搔癢
(GABA-RICH RICES COLONIZED WITH BACILLUS AMYLOLIQUEFACIENS STIMULATE COLLAGEN SYNTHESIS AND ATTENUATE TRICALCIUM PHOSPHATEINDUCED SKIN ITCHING)

相關論文

★ Intelligent nature-derived coordinative hydrogel incorporated with HRP as dressing for infected wounds	★ 表皮葡萄球菌在人類皮膚微生物總體對皮膚訊號與腦波訊號影響
★ 土壤微生物組體研究：藉由內生細菌誘導之高GABA含量水稻增加神經肽Y以及減輕小鼠焦慮	★ Fermentation of Leuconostoc mesenteroides reduces abdominal fat accumulation in high-fat diet mice
★ 選擇性發酵引發劑（SFI）觸發表皮葡萄球菌發酵以緩解UV-B誘導的自由基生成	★ Identify and characterize the fermenting and electrogenic skin bacteria using selective prebiotics
★ 有益微生物的真菌學和細菌學研究：在農業和人類健康中的應用	★ 人體皮膚致電微生物組通過調節鐵和自由基來減輕紫外線B引起的皮膚損傷。
★ 微生物組中的細菌作為治療人類疾病的生物療法	★ 皮膚表皮葡萄球菌作為電力活性菌以抑制痤瘡丙酸桿菌
★ 鼠李糖乳桿菌作為益生菌對抗 SARS-CoV-2 膜糖蛋白誘導的炎症	★ Flavin mononucleotide-based electricity production by Staphylococcus epidermidis alleviates SARS-CoV-2- Nucleocapsid Phosphoprotein-induced IL-6 expression
★ Profiling the Age-related Microbiome via Detection of Antibodies to Gut Bacteria	★ 人體汗水之乳酸鈉觸發人類皮膚益生菌之表皮葡萄球菌發酵及皮膚電導之應用
★ 5-甲基糠醛抑制L-乳酸葡萄球菌的發酵表皮葡萄球菌和雙乙酰產生：一種淺在的新型除臭劑靶向人體汗液中的细菌發酵	★ 甘油對於皮膚細菌和皮膚發電之影響

檔案

[Endnote RIS 格式]

[Bibtex 格式]

[相關文章]

[文章引用]

[完整記錄]

[館藏目錄]

至系統瀏覽論文 ( 永不開放)

摘要(中)

皮膚是人體最大也是最明顯的器官。瘙癢的皮膚，也稱為瘙癢，是一種刺激性和不舒服的感覺，常常伴隨著想要抓與刮皮膚的慾望以增強感受。慢性瘙癢會誘發發炎反應，持續造成白細胞介素(IL-6)的產生，進而導致表皮增生。另一方面，皺紋的形成是老化的一部分，與膠原蛋白降解有著關聯性。由成纖維細胞產生的第一型膠原(Col1)是人類皮膚中最主要的膠原類型。γ-氨基丁酸(GABA)是中樞神經系統中眾所周知的抑制性神經傳遞物質。近期的研究報導了GABA對傷口癒合的顯著影響及其作為免疫調節劑的功能。根據研究指出，植物生長促進內生細菌可以刺激植物的生長，並促進植物獲取營養、共生性能和脅迫耐受性增加的能力。

在這項研究內容，我們將水稻穀粒與解澱粉芽孢桿菌Bacillusamyloliquefaciens (BA)，一種非致病性土壤細菌，一起培養。我們的研究結果顯示，與BA細菌一同培養的水稻穀粒並沒有改變根和芽的長度，但GABA的含量卻有顯著的增加。經1mM和10mM GABA處理過的NIH 3T3細胞，其細胞中的Col1的蛋白質含量有明顯的提升。此外，GABA處理過的NIH 3T3細胞中的Col1 mRNA轉錄物的表現量會以以劑量依賴性的趨勢增加。

GABAB3-siRNA對GABA受體亞單位β-3 (GABAB3)的抑制顯著降低了NIH 3T3細胞中的Col1含量，說明了GABAB3介導了GABA誘導的Col1產生的過程。真皮內注射磷酸三鈣進入ICR小鼠背部皮膚能夠引起皮膚瘙癢。局部應用GABA或高GABA含量(BA菌共同培養生長)之稻米增加了Col1的產生，並減緩了磷酸三鈣誘導的瘙癢和IL-6含量之增加。

摘要(英)

Skin is the largest and most visible organ in human body. Itchy skin, also known as pruritus, is an irritating and an uncomfortable sensation that associated with the desire to scratch for enhancement the feeling. Chronic itching induces inflammation and sustains production of interleukin (IL)-6, which can lead to epidermal hyperplasia. On the other hand, the formation of wrinkles is part of the aging associated with degradation of the collagen. Type I collagen (Col1) produced by fibroblasts is the main type of the collagen in the human skin. Gamma-aminobutyric acid (GABA) is well known as an inhibitory neurotransmitter in the central nervous system. Recent studies have reported the significant effect of GABA on wound healing and its function as an immune-modulator. It is reported that plant growth-promoting endophytic bacteria can stimulate the growth, nutrient acquisition, symbiotic performance and stress tolerance of plants.
Here, we incubated rice grains with Bacillus amyloliquefaciens (BA), a non-pathogenic soil bacterium. Our results demonstrated that incubation of rice grains with BA bacteria did not change the lengths of roots and sprouts, but significantly increased the content of GABA. Treatment of 1mM and 10mM GABA significantly up-regulated the protein level of Col1 in NIH 3T3 cells. Furthermore, the expression of Col1 mRNA transcript was increased in NIH 3T3 cells when cells were treated with GABA in a dose-dependent manner.
Inhibition of GABA receptor subunit beta-3 (GABAB3) by GABAB3-siRNA considerably reduced the Col1 in NIH 3T3 cells, indicating the GABAB3 mediated the GABA-induced Col1 production. Intra-dermal injection of tri-calcium phosphate (Ca3(PO4)2) into the dorsal back skin of ICR mice caused itching. Topical application of GABA or GABA-rich (BA-colonized) rice onto mouse skin augmented the production of Col1 and attenuated the tri-calcium phosphate-induced decreases in itching and the level of IL-6.

關鍵字(中)

★ 液化澱粉芽孢桿菌
★ 搔癢
★ γ-氨基丁酸
★ 第一型膠原蛋白
★ 白細胞介素

關鍵字(英)

★ Bacillus amyloliquefaciens
★ GABA
★ IL-6
★ Itch
★ Type 1 collagen

論文目次

Contents
Chinese abstract i
English abstract ii
Acknowledgement iii
Contents iv
List of Figures iv
List of Tables vii
List of Abbreviations viii
Chapter 1: Introduction 1
1.1 Skin structure 1
1.2 Skin wrinkle 2
1.3 Collagen 4
1.4 Itching process 7
1.5 Gamma amino butyric acid 9
1.6 Nutrition of Rice 12
1.7 Bacillus amyloliquefaciens 13
Chapter 2: Materials and methods 16
2.1 Materials 16
2.2 Inoculation and bacterial culture 17
2.3 Cultivation of Rice 17
2.4 Determination of GABA content in Rice 17
2.5 Cell culture 19
2.6 Cell counting and treatment 20
2.7 Animal experiment 20
2.8 Protein extraction and measurement 21
2.8.1 Whole cell protein extraction from cell culture 21
2.8.2 Whole protein extraction from mice skin 21
2.8.3 Total protein concentration 21
2.9 Immunoblotting analysis 22
2.9.1 Poly-Acrylamide Gel Electrophoresis 22
2.9.2 Western blotting 23
2.9.3 Immunostaining 24
iv
2.9.4 Band densitometry 24
2.10 Elisa of Interleukin-6 25
2.11 Statistical analysis 25
Chapter 3: Results and discussion 26
3.1 Enhancement of the Col1 in NIH 3T3 cell by GABA compound 26
3.2 Knockdown 27
3.3 Enhancement of the Col1 in ICR mice by GABA compound 28
3.4 Rice sprout and root growth rate of with and without BA bacteria 29
3.5 GABA concentration of Rice 30
3.6 Collagen 1 expression of mice skin treated by GABA rich Rice 31
3.7 Mice skin scratching number treated by GABA compound and GABA rich Rice 32
3.8 IL-6 level of mice skin treated by GABA compound and GABA rich
Rice 33
Chapter 4: Conclusion and Future work 34
References 36

參考文獻

1. Randal.R Wicket and Marty.O Visscher, Structure and function of epidermal barrier. American Journal of Infection control, 2006.12(4):p.98-110.
2. Masayuki Yagi, Yoshikazu Yonei, Glycative stress and anti-aging. Glycative Stress Research, 2018.03: p.50-54.
3. Tortora.G, Grabowski.SR. Principles of anatomy and physiology. Harpercollins college publishers-1993
4. Genji Imokawa, Koichi Ishida, Biological Mechanisms Underlying the Ultraviolet Radiation-Induced Formation of Skin Wrinkling and Sagging I: Reduced Skin Elasticity, Highly Associated with Enhanced Dermal Elastase Activity, Triggers Wrinkling and Sagging, International Journal of Molecular Science, 2015.04(4): p.7754-7775.
5. J.Wang1, Thomas M. Michelitsch, A.Wunderlin, R.Mahadeva, Aging as a consequence of misrepair, 2009.03
6. Rebecca Small, Botulinum toxin Injection for facial wrinkle. American family physician, 2014.8 (3): p.168-175.
7. Kevin Connolly, Barbara Murdoch, Enhancement of Collagen Production in Skin Cells Via Cell Communication Signals. Metamorphosis, 2016.08
8. Nowsheen Goonoo, Dhanjay Jhurry, In vitro and in vivo cytocompatibility of electrospun nanofiber scaffolds for tissue engineering applications. 2014.07
9. M.Isabella, L.Barroso, M.Sanchez, Collagen: A review on its sources and potential cosmetic applications, Leading International dermatology Journal, 2017.11 p:20-26
10. Sylvie Ricard. Blum, The Collagen family, Cold spring harbor perspective in biology, 2011.01 (3)
11. Mitsuo Yamauchi, Marnisa Sricholpech, Lysine post-translational modifications of collagen. Essays In Biochemistry, 2012.5 (5): p.13-133.
12. Lilit Garibyan, G. Rheingold, A. Lerner, Understanding the pathophysiology of itch, Dermatol Ther. 2013.1 (2)
13. Weisshaar E., Matterne U, Epidemiology of itch in Itch, Mechanisms and Treatment, 2014. Chapter 2, :p9–18.
14. Weisshaar E, Dalgard F, Acta Derm Venereol.Epidemiology of itch: adding to the burden of skin morbidity, Advances in Dermatology and Venereology2009.3 (4):p.339-50.
15. L.Brasileiro, C.Barreto, Psychotropics in different causes of itch: systematic review with controlled studies, Official publication of the Brazilian Society of Dermatology.2016.11 (6)
16. E.Uehara, H.Hokazono, T.Sakaki, Effect of GABA on the expression of type 1 collagen gene in normal human dermal fibroblast, Bioscience, Biotechnology and Biochemistry, 2017.02 (2): p.376-379.
17. Roth RJ, Cooper JR, Bloom FE , The Biochemical Basis of Neuropharmacology.Oxford University Press.2013 :p.106.
18. Balemans.mg, Mans.D, Smit.I, Van Benthem, The influence of GABA on the synthesis of N-acetylserotonin, melatonin, O-acetyl-5-hydroxytryptophol and O-acetyl-5-methoxytryptophol in the pineal gland of the male Wistar rat. Nutrition development. 1983.2(1):p.151-60.
19. Philippe Nuss, Anxiety disorders and GABA neurotransmission: a disturbance of modulation. 2015 (11):p.165–175.
20. Ebadi.M, Wilt.S, Ramaley.R, Swanson.S, Mebus.C, The role of zinc and zinc-binding proteins in regulation of glutamic acid decarboxylase in brain. Progress in clinical and biological research. 1984;144A:p.255-75.
21. Mykkinnyn.M, uUSIoUKARI.m, Heikkil.J, Magnesium potentiation of the function of native and recombinant GABA(A) receptors. Neuroreport, 2001. 7 (10): p.175-179.
22. Sieghart W. Structure, pharmacology, and function of GABAA receptor subtypes. Adv Pharmacol. 2006:p.231-263.
23. S. Parnsakhorn* , J. Langkapin, Changes in physicochemical characteristics of germinated brown rice and brown rice during storage at various temperatures. Agricultural Engineering International: CIGR Journal. 2013.07(15): p. 293
24. D. Karladee, S. Suriyonga, γ-Aminobutyric acid (GABA) content in different varieties of brown rice during germination. 2012 (38): p. 13-17
25. Jaco.V, Daniel van der.L, Endophytic Bacteria and Their Potential Applications. Critical reviews in Plant Sciences 2018.12: p.583-606.
26. Chelius, M. K., & Triplett, E. W. Immunolocalization of dinitrogenase reductase produced by Klebsiella pneumoniae in association with Zea mays L. Applied and Environmental Microbiology, 2000.(2), 783-787.
27. Faeth, S. H., & Fagan, W. F. Fungal endophytes: Common host plant symbionts and uncommon mutualists. Integrative and Comparative Biology, 2002(2), 360-368
28. Shine.Km, Jamal M.Khaled, Naiyal.S, Renjiniemin.T, In-vitro antibacterial, antifungal, antioxidant and functional properties of Bacillus amyloliquefaciens. Annals of clinical microbiology and antimicrobials. 2015; 14: 9.
29. G. E. Demetrakopoulos, B. Linn and H. Amos, Biochem. Pharmac. 27, 373 1978. 15
30. . L. E. Saltzman, J. Moss, R. A. Berg, B. Horn and R. G. Crvstal. Biochem. J. 204. 25 11982)
31. A. Scum, S. and W. Harvey, Stimulation of human fibroblast collagen synthesis in vitro by y-aminobutyric acid. Biochemical Pharmacology, 1987 p. 1333-1335
32. Roach.JD, AguinaldoGT, Hughes FM, Gamma-aminobutyric acid inhibits synergistic interleukin-6 release but not transcriptional activation in astrocytoma cells. Neuroummunomodulation. 2008. 117-124

指導教授

黃俊銘(Eric.Chun-Ming Huang)

審核日期

2019-1-15

推文