DSpace community: 化學工程與材料工程研究所

115年化學工程與材料工程研究所-輸送現象與單元操作-入學考試題

title: 115年化學工程與材料工程研究所-輸送現象與單元操作-入學考試題 abstract: 國立中央大學圖書館-National Central University (Taiwan) Institutional Repository-考古題-工學院-化學工程與材料工程研究所-115年輸送現象與單元操作試題

115年化學工程與材料工程研究所-化工熱力學及化學反應工程-入學考試題

title: 115年化學工程與材料工程研究所-化工熱力學及化學反應工程-入學考試題 abstract: 國立中央大學圖書館-National Central University (Taiwan) Institutional Repository-考古題-工學院-化學工程與材料工程研究所-115年化工熱力學及化學反應工程試題

Synthesis of Biotinylated Silatrane via Huisgen Cycloaddition reaction for Bioconjugation Properties in Biosensors

title: Synthesis of Biotinylated Silatrane via Huisgen Cycloaddition reaction for Bioconjugation Properties in Biosensors abstract: 生物素是一種重要的生物分子，在細胞內多種關鍵代謝反應中扮演重要角色。特別是，生物素與親和素的結合是蛋白質與配體之間最強的非共價相互作用，展現出在生物感測器應用方面的潛力。然而，生物素在基底表面的非特異性結合會增加，導致生物感測器應用數據偏差。矽烷類化合物因其在水相中優異的化學穩定性以及透過跨環N→Si配位鍵調控的水解特性，成為一種有效的表面修飾化合物。這種結合可以控制水解並在表面形成自組裝單分子層。本研究首次報導了一種新型材料－生物素-矽烷（Bi-SiT），以矽烷和生物素為基礎的優異性能，利用Huisgen環加成反應合成。疊氮化物與炔烴的反應已成為合成具有高抗菌活性的1,2,3-三唑類化合物最有效的方法之一。此外，本研究將Bi-SiT與磺基甜菜鹼矽烷（SBSiT）混合，以提供一種可用於修飾的防污功能化表面。本研究透過疊氮矽烷（N=N=N）與生物素-乙炔（C≡C）的反應合成了生物素-矽烷。利用核磁共振氫譜（1H NMR）和傅立葉變換紅外光譜（FT-IR）證實了生物素-矽烷的結構。 Bi-SiT和SBSiT混合塗層的結果表明，塗覆在矽片上的塗層具有高親水性和均勻性，這突顯了其優異的防污和功能化性能。這些性能透過測量水接觸角、塗層厚度、X射線光電子能譜（XPS）和粗糙度得到驗證。此外，利用石英晶體微天平耗散監測（QCM-d）、橢圓偏振光譜和原子力顯微鏡（AFM）證實了表面上存在生物素蛋白。將矽烷環與生物素生物分子結合的生物素-矽烷將成為生物偶聯和生物感測器領域很有前景的材料.;Biotin is known as a biomolecule that plays important roles in a variety of critical metabolic reactions in cells. Especially, avidin-biotin binding is the strongest non-covalent interaction between a protein and ligand that demonstrates the potential for biosensor applications. However, biotin can be increased the non-specific binding on the surface of substrate that led to the mistaken data for biosensor applications. Silatranes are effective compounds for surface modification because of their excellent chemical stability in the presence of water and modulated hydrolysis characteristic through a transannular N→Si dative bond. The binding can controll the hydrolysis and form self-assemble monolayers film on the surface. This study reports for the first time that a new material biotin-silatrane (Bi-SiT) is synthesized based on the combination of the outstanding properties of silatrane and biotin using Huisgen cycloaddition chemistry. The reaction of azides with alkynes has become one of the most efficient methods to synthesize 1,2,3-triazoles that display a high antimicrobial activity. Additionally, Bi-SiT has been mixed with sulfobetaine silatrane (SBSiT) to provide an antifouling and functionalised surface for modification. In this study, the synthesis of biotin-silatrane was carried out by reacting azide-silatrane (N=N=N) and biotin-acetylene (C≡C). The structures of biotin-silatrane is confirmed by 1H nuclear magnetic resonance (NMR) and Fourier transform infrared (FT-IR) spectroscopies. The results for mixed coating between BiSiT and SBSiT showed the high hydrophilicity and uniform layers that emphasize the antifouling and funtionalised properties of the surface after coating on silicon wafer, determined by measuring water contact angle, thickness, X-ray Photoelectron Spectroscopy (XPS) and roughness. In addition, the detection of avidin on the surface was confirmed by Quartz crystal microbalance with dissipation monitoring (QCM-d), ellipsometry and Atomic Force Microscopy (AFM). Biotin-silatrane with the integration of silatrane ring and biotin biomolecule will be a promising material for bioconjugation, and biosensors.

Novel Trimethylamine N-oxide-Based Zwitterionic Hydrogels for Biomedical Application

title: Novel Trimethylamine N-oxide-Based Zwitterionic Hydrogels for Biomedical Application abstract: 由於具有高水合性、電荷中性以及優異的抗污行為，兩性離子水凝膠近年來在生醫材料、抗污塗層與生物感測器等領域受到廣泛關注。兩性離子材料能與水分子形成強烈作用並有效結合水分子，使其即使在複雜環境條件下，仍能展現優異的含水能力與抗污特性。在眾多兩性離子材料中，三甲胺N-氧化物（Trimethylamine N-oxide, TMAO）是一種天然存在於極端環境海洋生物中的滲透調節物質，因其獨特的水合行為與抗污特性，近年來引起研究者的高度關注。本論文提出一種完全以 TMAO 為基礎的兩性離子水凝膠，其由海鮮來源之 TMAO 單體與一種新穎且特別設計的兩性離子交聯劑——氧化甲基雙（ 2- 丙烯酰氧基乙基）胺（ Methyl-bis(2-acryloyloxyethyl)amine oxide, NOBA）所合成。該水凝膠展現良好的機械穩定性、優異的耐離子強度能力、卓越的抗污性能以及良好的抗凍特性，這些特性可歸因於強烈的水–聚合物交互作用以及水凝膠網絡中水分狀態的有效調控。此外，初步研究結果顯示，TMAO 水凝膠在魚類腐敗偵測方面具有潛在的生物標記應用價值，其特徵表現為水凝膠隨腐敗過程產生明顯膨潤並伴隨顏色變化，顯示其在食品安全生物感測領域具有良好的應用前景。;With the characteristics of increased hydration, charge neutrality, and strong antifouling behavior, zwitterionic hydrogels have attracted growing interest for use in biomedical materials, antifouling coatings, and biosensors. The unique ability of zwitterionic materials to interact with and bind water molecules leads to enhanced water retention and antifouling properties, even under complex environmental conditions. Among zwitterionic materials, trimethylamine N-oxide (TMAO), a naturally derived osmolyte found in marine organisms living in extreme conditions, has attracted interest in recent years due to its unique hydration and antifouling properties. This thesis presents the preparation of a fully TMAO-based zwitterionic hydrogel synthesized from a seafood-derived TMAO monomer and a novel, specifically designed zwitterionic cross-linker, Methyl-bis(2-acryloyloxyethyl)amine oxide (NOBA). This hydrogel exhibits favorable mechanical stability, strong resistance to ionic strength, excellent antifouling attributes, and good anti-freezing properties, which can be ascribed to the strong water-polymer interactions as well as the water state control in the hydrogel network. In addition, the initial results indicate that the TMAO hydrogel exhibits promising bio-tagging properties for detecting fish spoilage, as evidenced by visible expansion and color changes, suggesting strong potential for bio-sensing in food safety.