DSpace community: 生命科學系

Zelda potentiates morphogen activity by increasing chromatin accessibility

Thu, 23 Apr 2026 03:14:25 GMT

title: Zelda potentiates morphogen activity by increasing chromatin accessibility abstract: 出版者： Elsevier BV 出版日期： 2014-06 出處： Current Biology, 2014-06, Vol.24 (12), p.1341-1346 資源來源： Alma/SFX Local Collection 識別號： ISSN: 0960-9822 識別號： ISSN: 1879-0445 識別號： DOI: 10.1016/j.cub.2014.04.032

Zelda overcomes the high intrinsic nucleosome barrier at enhancers during Drosophila zygotic genome activation

Thu, 23 Apr 2026 03:14:24 GMT

title: Zelda overcomes the high intrinsic nucleosome barrier at enhancers during Drosophila zygotic genome activation abstract: 出版者：桃園市中壢區 : 國立中央大學出版日期： 2016 格式： 1冊 : 圖 ; 29公分. 附註：升等類別:薦升助理教授. 附註：封面日期:Auguest 1,2016. 附註：生醫理工學院/生命科學系. 附註：代表著作: Zelda overcomes the high intrinsic nucleosome barrier at enhancers during Drosophila zygotic genome activation/Yujia Sun, Chung-Yi Nien, Kai Chen, Hsiao-Yun Liu, Jeff Johnston, Julia Zeitlinger, and Christine Rushlow. Genome Res. 2015 Nov; 25(11): 1703–1714. doi: 10.1101/gr.192542.115 資源來源：圖書館目錄

Wnt3a signal pathways activate MyoD expression by targeting cis-elements inside and outside its distal enhancer

Thu, 23 Apr 2026 03:14:23 GMT

title: Wnt3a signal pathways activate MyoD expression by targeting cis-elements inside and outside its distal enhancer abstract: 摘要： Wnt proteins are secreted cytokines and several Wnts are expressed in the developing somites and surrounding tissues. Without proper Wnt stimulation, the organization of the dermomyotome and myotome can become defective. These Wnt signals received by somitic cells can lead to activation of Pax3/Pax7 and myogenic regulatory factors (MRFs), especially Myf5 and MyoD. However, it is currently unknown whether Wnts activate Myf5 and MyoD through direct targeting of their cis-regulatory elements or via indirect pathways. To clarify this issue, in the present study, we tested the regulation of MyoD cis-regulatory elements by Wnt3a secreted from human embryonic kidney (HEK)-293T cells. We found that Wnt3a activated the MyoD proximal 6.0k promoter (P6P) only marginally, but highly enhanced the activity of the composite P6P plus distal enhancer (DE) reporter through canonical and non-canonical pathways. Further screening of the intervening fragments between the DE and the P6P identified a strong Wnt-response element (WRE) in the upstream −8 to −9k region (L fragment) that acted independently of the DE, but was dependent on the P6P. Deletion of a Pax3/Pax7-targeted site in the L fragment significantly reduced its response to Wnt3a, implying that Wnt3a activates the L fragment partially through Pax3/Pax7 action. Binding of β-catenin and Pax7 to their target sites in the DE and the L fragment respectively was also demonstrated by ChIP. These observations demonstrated the first time that Wnt3a can directly activate MyoD expression through targeting cis-elements in the DE and the L fragment. 其他題名： Biosci Rep 出版者： England: Portland Press Ltd 出版日期： 2015-03-18 出處： Bioscience Reports, 2015-03, Vol.35 (2), p.e00180- 資源來源： Agricultural & Environmental Science Collection 版權： 2015 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. 2015 識別號： ISSN: 0144-8463 識別號： ISSN: 1573-4935 識別號： EISSN: 1573-4935 識別號： DOI: 10.1042/bsr20140177 識別號： PMID: 25651906

VDR and VDBP genes polymorphisms associated with susceptibility to tuberculosis in a Han Taiwanese population

Thu, 23 Apr 2026 03:14:22 GMT

title: VDR and VDBP genes polymorphisms associated with susceptibility to tuberculosis in a Han Taiwanese population abstract: 摘要： The active metabolite (1, 25-dihydroxycholecalciferol) of vitamin D (25-hydroxycholecalciferol) leads to the activation of macrophages and the deficiency of vitamin D seems to be involved in the risk of tuberculosis (TB). The effects of vitamin D are exerted by interaction with the vitamin D receptor (VDR) and vitamin D receptor binding protein (VDBP) may be influenced by polymorphisms in the VDR and VDBP genes. In this study, variation in the VDR and VDBP genes was investigated in a Taiwanese population with TB. We typed four VDR polymorphisms of restriction endonuclease sites for ApaI, TaqI, BsmI, and FokI and three VDBP polymorphisms—Thr420Lys, Asp416Glu, and Cys299Cys—in 198 patients with TB and 170 healthy volunteers. VDR TaqI, VDR BsmI, and VDBP Asp416Glu were significantly associated with TB susceptibility. Odd ratios of risk genotypes of the above three polymorphisms were 2.16 (95% confidence interval 1.01, 4.65), 2.14 (95% confidence interval 1.06, 4.31), and 2.24 (95% confidence interval 1.04, 4.80), respectively. VDBP haplotype analysis showed Gc1f carriers associated to TB. The polymorphisms in the VDR and VDBP genes appeared to be responsible for host susceptibility to human TB in a Taiwanese population. 其他題名： J Microbiol Immunol Infect 出版者： England: Elsevier B.V 出版日期： 2016-10-01 出處： Journal of microbiology, immunology and infection, 2016-10, Vol.49 (5), p.783-787 資源來源： Elsevier ScienceDirect Journals Complete 版權： 2016 版權： Copyright © 2016. Published by Elsevier B.V. 識別號： ISSN: 1684-1182 識別號： ISSN: 1995-9133 識別號： EISSN: 1995-9133 識別號： DOI: 10.1016/j.jmii.2015.12.008 識別號： PMID: 26869016