| 摘要: | 摘要： Mosquitoes act as vectors of devastating pathogens and parasites, representing a key threat for millions of humans and animals worldwide. Eco-friendly control tools are urgently required. We proposed a novel method of fern-mediated biosynthesis of silver nanoparticles (AgNP) using Dicranopteris linearis, acting as a reducing and capping agent. AgNP were characterized by UV–vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), zeta potential and particle size analysis. In mosquitocidal assays, the LC50 of D. linearis extract against Aedes aegypti ranged from 165.213 (larva I) to 255.055ppm (pupa). LC50 of D. linearis-synthesized AgNP ranged from 18.905 (larva I) to 29.328ppm (pupa). In the field, the application of D. linearis extract and AgNP (10×LC50) led to 100% larval reduction after 72h. Smoke toxicity experiments conducted against A. aegypti adults showed that D. linearis leaf-, stem- and root-based coils evoked mortality rates comparable to the permethrin-based positive control (58%, 47%, 34%, and 48% respectively). In ovicidal experiments, egg hatchability was reduced by 100% after treatment with 25ppm of AgNP and 300ppm of D. linearis extract. Interestingly, oviposition deterrent assays highlighted that 100ppm of fern extract reduced oviposition rates of more than 65%, while 10ppm of fern-fabricated AgNP reduced oviposition rates of more than 70% in A. aegypti (OAI were −0.52 and −0.55, respectively). Overall, our results highlighted that D. linearis-synthesized AgNP could be useful candidates to develop nano-formulated oviposition deterrents effective against dengue vectors. [Display omitted] •Mosquito vectors represent a threat for millions of humans and animals worldwide.•Little is currently known on the effectiveness of nano-formulated oviposition deterrents.•We used the fern Dicranopteris linearis to fabricate Ag nanoparticles.•Nanoparticles were characterized by UV–vis, FTIR, SEM, EDX, XRD, zeta potential and size analysis.•10ppm of Ag nanoparticles reduced Aedes aegypti oviposition of more than 70% (OAI=−0.55).
其他題名： Res Vet Sci
出版者： England: Elsevier Ltd
出版日期： 2016-12
出處： Research in Veterinary Science, 2016-12, Vol.109, p.40-51
版權： 2016 Elsevier Ltd
版權： Copyright © 2016 Elsevier Ltd. All rights reserved.
版權： Copyright Elsevier Limited Dec 01, 2016
識別號： ISSN: 0034-5288
識別號： ISSN: 1532-2661
識別號： EISSN: 1532-2661
識別號： DOI: 10.1016/j.rvsc.2016.09.012
識別號： PMID: 27892872 |
