Ferric plasmonic nanoparticles, aptamers, and magnetofluidic chips: Toward the development of diagnostic surface-enhanced Raman spectroscopy assays

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題名:	Ferric plasmonic nanoparticles, aptamers, and magnetofluidic chips: Toward the development of diagnostic surface-enhanced Raman spectroscopy assays
作者:	黃柏榮;Marks, Haley;Huang, Po-Jung;Mabbott, Samuel;Graham, Duncan;Kameoka, Jun;Coté, Gerard
貢獻者:	工學院化學工程與材料工程學系
關鍵詞:	Aptamers, Nucleotide - chemistry;Equipment Design;Magnetite Nanoparticles - chemistry;Molecular Diagnostic Techniques;Research Papers: Sensing;Silver - chemistry;Spectrum Analysis, Raman - methods
日期:	2016-12-01
上傳時間:	2026-04-21 14:57:12 (UTC+8)
出版者:	SPIE;United States: Society of Photo-Optical Instrumentation Engineers
摘要:	摘要： Conjugation of aptamers and their corresponding analytes onto plasmonic nanoparticles mediates the formation of nanoparticle assemblies: molecularly bound nanoclusters that cause a measurable change in the colloid's optical properties. The optimization of a surface-enhanced Raman spectroscopy (SERS) competitive binding assay utilizing plasmonic "target" and magnetic "probe" nanoparticles for the detection of the toxin bisphenol-A (BPA) is presented. These assay nanoclusters were housed inside three types of optofluidic chips patterned with magnetically activated nickel pads, in either a straight or array pattern. Both Fe2O3 and Fe2CoO4 were compared as potential magnetic cores for the silver-coated probe nanoparticles. We found that the AgFe2O3 particles were, on average, more uniform in size and more stable than AgFe2CoO4, whereas the addition of cobalt significantly improved the collection time of particles. Using Raman mapping of the assay housed within the magnetofluidic chips, it was determined that a 1×5 array of 50 μm square nickel pads provided the most uniform SERS enhancement of the assay (coefficient of variation ∼25%) within the magnetofluidic chip. Additionally, the packaged assay demonstrated the desired response to BPA, verifying the technology's potential to translate magnetic nanoparticle assays into a user-free optical analysis platform. 其他題名： J. Biomed. Opt 出版者： United States: Society of Photo-Optical Instrumentation Engineers 出版日期： 2016-12-01 出處： Journal of biomedical optics, 2016-12, Vol.21 (12), p.127005-127005 版權： 2016 Society of Photo-Optical Instrumentation Engineers (SPIE) 版權： 2016 Society of Photo-Optical Instrumentation Engineers (SPIE) 2016 Society of Photo-Optical Instrumentation Engineers 識別號： ISSN: 1083-3668 識別號： ISSN: 1560-2281 識別號： EISSN: 1560-2281 識別號： DOI: 10.1117/1.JBO.21.12.127005 識別號： PMID: 27997017
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