| 摘要: | 摘要： We performed a BLAST search of the rice database and screened four group 3 late embryogenesis abundant genes ( OsG3LEA-47.3 , OsG3LEA-41.9 , OsG3LEA-20.5 , and OsG3LEA-24.5 ) that shared characteristics of canonical G3LEAs such as multiple copies of consensus motif, hydrophilic, structural intrinsic disorder, thermostability, abscisic acid (ABA)-responsiveness, and high G and C content in gene sequence. Under nonstress conditions, OsG3LEA-20.5 and OsG3LEA-24.5 were constitutively expressed, whereas OsG3LEA-47.3 and OsG3LEA-41.9 were expressed in a stage- or tissue-specific manner. Transcripts of OsG3LEA-20.5 and OsG3LEA-24.5 accumulated under salt, ABA, and cold stress treatment. By contrast, OsG3LEA-47.3 and OsG3LEA-41.9 showed less responsiveness to stress in tillering and heading stages, respectively. All genes showed enhanced transcript levels after pollination and embryo development. To investigate the functions, we overexpressed OsG3LEA-47.3 , OsG3LEA-41.9 , OsG3LEA-20.5 , and OsG3LEA-24.5 in Arabidopsis (47.3-ox, 41.9-ox, 20.5-ox, and 24.5-ox). Analysis of tolerance to drought stress revealed higher recovery after 14 days of dehydration treatment for 47.3-ox or 41.9-ox than the wild type (WT), 20.5-ox, or 24.5-ox. In addition, 47.3-ox and 24.5-ox plants showed higher survival than WT, 41.9-ox, and 20.5-ox plants under heat treatment, which induced similar expression patterns of heat shock protein genes in WT, 47.3-ox, 41.9-ox, 20.5-ox, and 24.5-ox plants. In vitro chaperone activity with the model substrate citrate synthase was comparable between OsG3LEA-47.3 and OsHSP16.9A, a rice molecular chaperone providing thermoprotection in vivo and in vitro. These results suggest that rice G3LEAs share many physical and biological features but function in contrasting ways.
其他題名： Plant Mol Biol Rep
出版者： New York: Springer US
出版日期： 2016-10
出處： Plant molecular biology reporter, 2016-10, Vol.34 (5), p.1003-1015
資源來源： Agricultural & Environmental Science Collection
版權： Springer Science+Business Media New York 2016
識別號： ISSN: 0735-9640
識別號： EISSN: 1572-9818
識別號： DOI: 10.1007/s11105-016-0983-1 |
