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| 題名: | Distinct role of Arabidopsis mitochondrial P-type pentatricopeptide repeat protein-modulating editing protein, PPME, in nad1 RNA editing |
| 作者: | 謝明勳;Leu, Kuan-Chieh;Hsieh, Ming-Hsiun;Wang, Huei-Jing;Hsieh, Hsu-Liang;Jauh, Guang-Yuh |
| 貢獻者: | 生醫理工學院生命科學系 |
| 關鍵詞: | amino acids;Arabidopsis;Arabidopsis - genetics;Arabidopsis - growth & development;Arabidopsis Proteins - chemistry;Arabidopsis Proteins - genetics;Arabidopsis Proteins - metabolism;Arabidopsis thaliana;complex I activity;cytosine;deamination;editing;Electron Transport Complex I - metabolism;gene expression;Gene Expression Regulation, Developmental;Gene Expression Regulation, Plant;generators (equipment);genomics;growth and development;homozygosity;mitochondria;mitochondrial RNA;mutants;Mutation;nad1;NADH Dehydrogenase - genetics;nucleotides;PPR;Protein Binding;Research Paper;RNA Editing;RNA, Messenger - metabolism;RNA, Plant - metabolism |
| 日期: | 2016-06-02 |
| 上傳時間: | 2026-04-23 11:12:19 (UTC+8) |
| 出版者: | Landes Bioscience;United States: Taylor & Francis |
| 摘要: | 摘要： The mitochondrion is an important power generator in most eukaryotic cells. To preserve its function, many essential nuclear-encoded factors play specific roles in mitochondrial RNA metabolic processes, including RNA editing. RNA editing consists of post-transcriptional deamination, which alters specific nucleotides in transcripts to mediate gene expression. In plant cells, many pentatricopeptide repeat proteins (PPRs) participate in diverse organellar RNA metabolic processes, but only PLS-type PPRs are involved in RNA editing. Here, we report a P-type PPR protein from Arabidopsis thaliana, P -type P PR- M odulating E diting (PPME), which has a distinct role in mitochondrial nad1 RNA editing via RNA binding activity. In the homozygous ppme mutant, cytosine (C)-to-uracil (U) conversions at both the nad1-898 and 937 sites were abolished, disrupting Arg 300 -to-Trp 300 and Pro 313 -to-Ser 313 amino acid changes in the mitochondrial NAD1 protein. NAD1 is a critical component of mitochondrial respiration complex I; its activity is severely reduced in the homozygous ppme mutant, resulting in significantly altered growth and development. Both abolished RNA editing and defective complex I activity were completely rescued by CaMV 35S promoter- and PPME native promoter-driven PPME genomic fragments tagged with GFP in a homozygous ppme background. Our experimental results demonstrate a distinct role of a P-type PPR protein, PPME, in RNA editing in plant organelles.
其他題名： RNA Biol
出版者： United States: Taylor & Francis
出版日期： 2016-06-02
出處： RNA Biology, 2016-06, Vol.13 (6), p.593-604
資源來源： Taylor & Francis Open Access
版權： 2016 The Author(s). Published with license by Taylor & Francis Group, LLC © Kuan-Chieh Leu, Ming-Hsiun Hsieh, Huei-Jing Wang, Hsu-Liang Hsieh, and Guang-Yuh Jauh 2016
版權： 2016 The Author(s). Published with license by Taylor & Francis Group, LLC 2016 The Author(s)
識別號： ISSN: 1547-6286
識別號： ISSN: 1555-8584
識別號： EISSN: 1555-8584
識別號： DOI: 10.1080/15476286.2016.1184384
識別號： PMID: 27149614 |
| 顯示於類別: | [生命科學系] 期刊論文
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